My watch list
my.chemeurope.com  
Login  

Protein structure prediction



Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry. Its aim is the prediction of the three-dimensional structure of proteins from their amino acid sequences, sometimes including additional relevant information such as the structures of related proteins. In other words, it deals with the prediction of a protein 's tertiary structure from its primary structure. Protein structure prediction is of high importance in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment.

Contents

Overview

The practical role of protein structure prediction is now more important than ever. Massive amounts of protein sequence data are produced by modern large-scale DNA sequencing efforts such as the Human Genome Project. Despite community-wide efforts in structural genomics, the output of experimentally determined protein structures — typically by time-consuming and relatively expensive X-ray crystallography or NMR spectroscopy — is lagging far behind the output of protein sequences.

A number of factors exist that make protein structure prediction a very difficult task. The two main problems are that the number of possible protein structures is extremely large, and that the physical basis of protein structural stability is not fully understood. As a result, any protein structure prediction method needs a way to explore the space of possible structures efficiently (a search strategy), and a way to identify the most plausible structure (an energy function).

In comparative structure prediction, the search space is pruned by the assumption that the protein in question adopts a structure that is reasonably close to the structure of at least one known protein. In de novo or ab initio structure prediction, no such assumption is made, which results in a much harder search problem. In both cases, an energy function is needed to recognize the native structure, and to guide the search for the native structure. Unfortunately, the construction of such an energy function is to a great extent an open problem.

Direct simulation of protein folding in atomic detail, via methods such as molecular dynamics with a suitable energy function, is typically not tractable due to the high computational cost, despite the efforts of distributed computing projects such as Folding@home. Therefore, most de novo structure prediction methods rely on simplified representations of the atomic structure of proteins.

The above mentioned issues apply to all proteins, including well-behaving, small, monomeric proteins. In addition, for specific proteins (such as for example multimeric proteins and disordered proteins), the following issues also arise:

  • Some proteins require stabilisation by additional domains or binding partners to adopt their native structure. This requirement is typically unknown in advance and difficult to handle by a prediction method.
  • The tertiary structure of a native protein may not be readily formed without the aid of additional agents. For example, proteins known as chaperones are required for some proteins to properly fold. Other proteins cannot fold properly without modifications such as glycosylation.
  • A particular protein may be able to assume multiple conformations depending on its chemical environment.
  • The biologically active conformation may not be the most thermodynamically favorable.

Due to the increase in computer power, and especially new algorithms, much progress is being made to overcome these problems. However, routine de novo prediction of protein structures, even for small proteins, is still not achieved.

Ab initio protein modelling

Ab initio- or de novo- protein modelling methods seek to build three-dimensional protein models "from scratch", i.e., based on physical principles rather than (directly) on previously solved structures. There are many possible procedures that either attempt to mimic protein folding or apply some stochastic method to search possible solutions (i.e., global optimization of a suitable energy function). These procedures tend to require vast computational resources, and have thus only been carried out for tiny proteins. To predict protein structure de novo for larger proteins will require better algorithms and larger computational resources like those afforded by either powerful supercomputers (such as Blue Gene or MDGRAPE-3) or distributed computing (such as Folding@home, the Human Proteome Folding Project and Rosetta@Home). Although these computational barriers are vast, the potential benefits of structural genomics (by predicted or experimental methods) make ab initio structure prediction an active research field.

Comparative protein modelling

Comparative protein modelling uses previously solved structures as starting points, or templates. This is effective because it appears that although the number of actual proteins is vast, there is a limited set of tertiary structural motifs to which most proteins belong. It has been suggested that there are only around 2000 distinct protein folds in nature, though there are many millions of different proteins.

These methods may also be split into two groups:

  • Homology modelling is based on the reasonable assumption that two homologous proteins will share very similar structures. Because a protein's fold is more evolutionarily conserved than its amino acid sequence, a target sequence can be modeled with reasonable accuracy on a very distantly related template, provided that the relationship between target and template can be discerned through sequence alignment. It has been suggested that the primary bottleneck in comparative modelling arises from difficulties in alignment rather than from errors in structure prediction given a known-good alignment.[1] Unsurprisingly, homology modelling is most accurate when the target and template have similar sequences.
  • Protein threading[2] scans the amino acid sequence of an unknown structure against a database of solved structures. In each case, a scoring function is used to assess the compatibility of the sequence to the structure, thus yielding possible three-dimensional models. This type of method is also known as 3D-1D fold recognition due to its compatibility analysis between three-dimensional structures and linear protein sequences. This method has also given rise to methods performing an inverse folding search by evaluating the compatibility of a given structure with a large database of sequences, thus predicting which sequences have the potential to produce a given fold.

Side chain geometry prediction

Even structure prediction methods that are reasonably accurate for the peptide backbone often get the orientation and packing of the amino acid side chains wrong. Methods that specifically address the problem of predicting side chain geometry include dead-end elimination and the self-consistent mean field method. Both discretize the continuously varying dihedral angles that determine a side chain's orientation relative to the backbone into a set of rotamers with fixed dihedral angles. The methods then attempt to identify the set of rotamers that minimize the model's overall energy. Rotamers are the side chain conformations with low energy. Such methods are most useful for analyzing the protein's hydrophobic core, where side chains are more closely packed; they have more difficulty addressing the looser constraints and higher flexibility of surface residues.[3]

Software

MODELLER is a popular software tool for producing homology models using methodology derived from NMR spectroscopy data processing. SwissModel provides an automated web server for basic homology modeling. A common software tool for protein threading is 3D-PSSM. The basic algorithm for threading is described in[2] and is fairly straightforward to implement.

TIP is a knowledgebase of STRUCTFAST[4] models and precomputed similarity relationships between sequences, structures, and binding sites.

A very recent review of currently popular software for structure prediction can be found at.[5] A partial list of web servers and available tools is maintained here.

Several distributed computing projects concerning protein structure prediction have also been implemented, such as the Folding@home, Rosetta@home, Human Proteome Folding Project, Predictor@home and TANPAKU.

Protein-protein complexes

In the case of complexes of two or more proteins, where the structures of the proteins are known or can be predicted with high accuracy, protein-protein docking methods can be used to predict the structure of the complex. Information of the effect of mutations at specific sites on the affinity of the complex helps to understand the complex structure and to guide docking methods.

See also

References

  1. ^ Zhang Y and Skolnick J (2005). "The protein structure prediction problem could be solved using the current PDB library". Proc Natl Acad Sci USA 102 (4): 1029-1034. Entrez PubMed 15653774.
  2. ^ a b Bowie JU, Luthy R, Eisenberg D (1991). "A method to identify protein sequences that fold into a known three-dimensional structure". Science 253 (5016): 164-170. Entrez PubMed 1853201.
  3. ^ Voigt CA, Gordon DB, Mayo SL (2000). "Trading accuracy for speed: A quantitative comparison of search algorithms in protein sequence design". J Mol Biol 299 (3): 789-803. Entrez PubMed 10835284.
  4. ^ Debe DA, Danzer JF, Goddard WA, Poleksic A (2006). "STRUCTFAST: Protein sequence remote homology detection and alignment using novel dynamic programming and profile-profile scoring". Proteins 64: 960-967. Entrez PubMed 16786595.
  5. ^ Nayeem A, Sitkoff D, Krystek S Jr (2006). "A comparative study of available software for high-accuracy homology modeling: From sequence alignments to structural models". Protein Sci 15: 808-824. Entrez PubMed 16600967.


Further reading

  • Bonneau R, Baliga NS, Deutsch EW, Shannon P, Hood L (2004). "Comprehensive de novo structure prediction in a systems-biology context for the archaea Halobacterium sp. NRC-1". Genome Biology, 5(8):R52-68.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Protein_structure_prediction". A list of authors is available in Wikipedia.
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE