Cell Normalization Using Adhesive Micropatterns

The most common cell culture method to study cell behavior under different stimuli, is the Petri Dish or its variants in multi-well plates. These systems are easy to use and versatile but still suffer from large cell variability. Reducing cell variability and developing efficient image analysis methodologies are key in reaching high quality and reproducible results both in fundamental cell biology and in High Content Analysis assays. This hands-on course will introduce you to a powerful technology based on CYTOO’s adhesive micropatterns which normalize cell architecture down to their internal organization. The course will focus on the practical aspects of cell normalization using micropatterns in a model drug assay.

The theoretical part includes:

Control of cell architecture and behavior by cell microenvironment engineering
Introduction to CYTOO’s adhesive micropatterns
Automated image acquisition and image processing of micropatterned cells
Live Cell Imaging using micropatterns
Examples of applications using various micropatterns
Design of custom micropatterns adapted to your biological questions
Adhesive micropatterns in High Content Screening and Drug Discovery
Presentation of a case study
Reference CellTM concept
The practical part includes:

Cell seeding experiments on adhesive micropatterns
Microscope observation of cell spreading on micropatterns
Acquisition of fluorescence microscope images on the fixed and stained demo samples
Automated image processing and analysis (using ImageJ)

Target Group
Technical and scientific staff members with initial cell culture experience interested in cell normalization and quantitative cell imaging and analysis; developers of HCA cell-based assays.

Dates
PA3121 22.06.2012
Beginning of the course: 9:00 am
End of the course: approx. 4:30 pm

The fee for 1 day training amounts to 389,- € (plus VAT)