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Cloning Strategiesin Heidelberg from 01.10.2012 - 02.10.2012
In this one-and-a-half-day theory course, basic methods and strategies for the cloning of DNA fragments are explained in detail.
Topics covered include:
Cloning strategies: successful and efficient cloning
Vectors: possibilities and choice (standard cloning vectors, eukaryotic expression vectors, inducible systems, etc.)
Enzymes: isochizomeres, compatible ends, suitable standard enzymes, fill-in and blunt end, dephosphorylation, etc.
Restriction digest, modification, and preparation
Ligation: stoichiometry and discussion of different methods
Transformation: competent bacteria, generation, and efficiency control
Selection: resistance to antibiotics, blue-white-selection using ß- galactosidase genes, analysis and control of results
Clone analysis, mini and maxi preps
DNA quality and purity requirements: gel purification and isolation
Removal of stop codons
Tips and tricks for difficult cloning
Technicians and researchers with previous knowledge of molecular biology who want to get a comprehensive overview of cloning strategies and want to optimize their cloning protocols.
PA4591 01.10.2012 - 02.10.2012
Beginning of the course on the first day: 9:00 am
End of the course on the last day: approx. 1:00 pm
The fee for 1.5 days training amounts to 579,- € (plus VAT)
|Event Date:||01.10.2012 to 02.10.2012|
Frau Daniela Pfeifer
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