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Exceeding Industry Standards with Thermo Scientific Nanopure Water

by Thermo Fisher Scientific, Inc.
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Having developed new water quality specifications for the Thermo Scientific Nanopure Life Science water purification systems, Thermo Fisher Scientific can now offer you reagent grade laboratory water which exceeds the industry standards.

The system was evaluated to assess its effectiveness at eliminating bacteria, pyrogens and nucleases from a sample using highly sensitive test protocols. Resulting data indicated that nanopure-purified water can be used in applications such as PCR, plasmid preparation and in vitro translations, which are extremely sensitive to impurities.

Since water has such a wide variety of uses within different life science laboratories, from autoclaving, humidification and glassware rinsing to serial dilution and sample preparation, it is imperative that the water produced is free from any contaminants that will have a negative impact on your resulting data. These newly developed specifications help to ensure your water is truly pure, helping you to accurately maintain experimental integrity.

Testing of feed water filtered by the nanopure system showed pyrogen levels of <0.001 EU/ ml and bacterial levels of <0.01 CFU/ ml. Further testing for the presence of nucleases demonstrated that the nanopure system is capable of producing <0.003 ng/ ml of RNase and <0.4 pg/ µl of DNase.

These results exceed current industry standards for Type I water provided by institutions such as the American Society of Testing and Materials (ASTM) and the Clinical and Laboratory Standards Institute-Clinical Laboratory Reagent Water (CLSI®-CLRW). As the highest purity grade, Type I water is suitable for even the most sensitive analytical applications, guaranteeing that your results are accurate.

Unlike previous Diethylpyrocarbonate (DEPC) methods, the Thermo Scientific Nanopure Life Science unit provides a constant, on-demand source of nuclease-free water. The use of ultrafiltration provides an easy and fast method of removing nucleases from aqueous solution. In contrast, other methods such as DEPC and autoclaving are time-consuming, and DEPC is a suspected carcinogen with potentially toxic effects.

The incorporation of an ultrafilter directly within the main circulation of the system facilitates the simple removal of pyrogens, bacteria, and nucleases. Furthermore, it enables continuous rinsing of the filter, which not only provides additional protection from potential sources of contamination, but also expands the life-span of the filter by up to two years. This cost-effective pure water system produces extremely high-quality, nuclease- and pyrogen-free water, as and when it is required.

To find out more about the new specifications of the Thermo Scientific Nanopure Life Science pure water system, and to view or download the Thermo Scientific Waterbook, visit our product web page.

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