Improving the efficiency of collagen coating of plates and flasks

INTEGRA has released an application note that describes a new protocol using its DOSE IT peristaltic pump that significantly improves the efficiency of collagen coating of plates and flasks.

Coating of dishes or tissue culture flasks with extra cellular matrix proteins like Collagen or Fibronectin is a common procedure in laboratories involved in cell culture. However, coating large numbers of disposables is a tedious and time-consuming task. This application note describes how using a DOSE-IT peristaltic pump results in 40% time-savings compared to a regular pipette controller and at the same time helps to reduce excessive strain caused by this repetitive work.

DOSE IT is an intuitive programmable peristaltic pump that simplifies the dispensing of cell culture media, buffers and other solutions for volumes ranging from 0.1mL to 2500mL. DOSE IT features a very small footprint and weighs only 3.5Kg making it a perfect device for bench-top or in-hood dispensing applications. Programming the DOSE IT is fast and easy through a multilingual user interface (English, Spanish, German, French and Italian). The instrument's pump head accommodates the use of different tubing diameters (1-8mm) allowing a wide range of volumes to be dispensed with optimal speed and precision. A built-in print capability supports the capture of program details for record keeping and quality control. Many accessories are available to support a variety of dispensing applications.

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