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Tosoh Bioscience, the manufacturer of the well-known TSKgel Amide-80 column, expands the selectivity range of HILIC solutions by a new, amino-type HILIC column with high stability.
The new HILIC phase is based on a 3 µm silica particle with 100 Å pores, which is treated with a special endcapping procedure. Amino groups are introduced step wisely after endcapping. The amino groups act as HILIC functional groups without any peak splits. Due to a high ligand density and large surface area TSKgel NH2-100V 3 µm columns show high retention for very polar compounds.
Hydrophilic interaction liquid chromatography (HILIC) is the preferred method for the separation of polar compounds. While TSKgel Amide-80 columns became the industrial standard for the separation of glycans by HILIC or HILIC-MS, the new column is well suited for all separations requiring amino type functional groups. In contrast to conventional silica-based amino phases the new column offers expanded stability under HILIC conditions, the prerequisite for reproducible and reliable results.
TSKgel NH2-100 columns can be applied for the analysis of all types of hydrophilic compounds like carbohydrates, peptides or polar metabolites.
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