Nature Protocols 9, 2823 (2014).
Authors: Thomas O Auer, Karine Duroure, Jean-Paul Concordet & Filippo Del Bene
Here we present a protocol for the conversion of eGFP-transgenic zebrafish lines into lines expressing Gal4 from the same locus. This ... more
The RhoGEF (Rho GTPase guanine-nucleotide-exchange factor) domain of AKAP-Lbc (A-kinase-anchoring protein-Lbc, also known as AKAP13) catalyses nucleotide exchange on RhoA and is involved in the development of cardiac hypertrophy. The RhoGEF activity of AKAP-Lbc has also been implicated in c ... more
Class II/III PI3Ks (phosphoinositide 3-kinases) produce the PtdIns(3)P lipid that is involved in intracellular vesicular trafficking. In contrast with class I PI3Ks, the potential signalling roles of class II/III PI3Ks are poorly understood. In a recent article in the Biochemical Journal, B ... more
Nature Protocols 9, 2771 (2014).
Authors: Paolo Marcatili, Pier Paolo Olimpieri, Anna Chailyan & Anna Tramontano
Antibodies (or immunoglobulins) are crucial for defending organisms from pathogens, but they are also key players in many medical, d ... more
Nature Protocols 9, 2725 (2014).
Authors: Rachel H Giles, Henry Ajzenberg & Peter K Jackson
We have developed a novel 3D cell culture model that uses mouse inner-medullary collecting duct (mIMCD3) cells to generate epithelial spheroids. This mod ... more