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Mechanical isolation, and measurement of force and myoplasmic free [Ca2+] in fully intact single skeletal muscle fibers

Nature Protocols 12, 1763 (2017). doi:10.1038/nprot.2017.056

Authors: Arthur J Cheng & Håkan Westerblad

Mechanical dissection of single intact mammalian skeletal muscle fibers permits real-time measurement of intracellular properties and contractile function of living fibers. A major advantage of mechanical over enzymatic fiber dissociation is that single fibers can be isolated with their tendons remaining attached, which allows contractile

Authors:   Arthur J Cheng; Håkan Westerblad
Journal:   Nature Protocols
Volume:   12
edition:   9
Year:   2017
Pages:   1763
DOI:   10.1038/nprot.2017.056
Publication date:   01-Sep-2017
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