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Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI

Nature Protocols 12, 2050 (2017). doi:10.1038/nprot.2017.081

Authors: Anupama Lakshmanan, George J Lu, Arash Farhadi, Suchita P Nety, Martin Kunth, Audrey Lee-Gosselin, David Maresca, Raymond W Bourdeau, Melissa Yin, Judy Yan, Christopher Witte, Dina Malounda, F Stuart Foster, Leif Schröder & Mikhail G Shapiro

Gas vesicles (GVs) are a unique class of gas-filled protein nanostructures that are detectable at subnanomolar concentrations and whose physical properties allow them to serve as highly sensitive imaging agents for ultrasound and MRI. Here we provide a protocol for isolating GVs from native and

Authors:   Anupama Lakshmanan; George J Lu; Arash Farhadi; Suchita P Nety; Martin Kunth; Audrey Lee-Gosselin; David Maresca; Raymond W Bourdeau; Melissa Yin; Judy Yan; Christopher Witte; Dina Malounda; F Stuart Foster; Leif Schröder; Mikhail G Shapiro
Journal:   Nature Protocols
Volume:   12
edition:   10
Year:   2017
Pages:   2050
DOI:   10.1038/nprot.2017.081
Publication date:   01-Oct-2017
Facts, background information, dossiers
  • gas
  • Witte
  • ultrasound
  • imaging agents
  • contrast agents
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