The dry rhizome of Acorus tatarinowii Schott is a traditional Chinese medicine (TCM) which has been used to treat many diseases, for example epilepsy, for thousands of years. In this work, microwave distillation and simultaneous solid-phase microextraction (MD-SPME) were used for analysis of the essential oil in the fresh leaves of Acorus tatarinowii. Isolation, extraction, and concentration of the volatile constituents of the leaves can be completed rapidly, in a single step, by use of MD-SPME; the compounds can then be analyzed by gas chromatography-mass spectrometry (GC-MS). MD-SPME conditions, including microwave power, irradiation time, and SPME fiber coating, were studied. By use of MD-SPME-GC-MS twenty-nine compounds were identified, for the first time, in the essential oil of the plant leaves; the compounds were the same as those in the rhizomes. This suggests the leaves of the plant might be used as a TCM. To demonstrate the feasibility of the method, conventional steam distillation (SD) was also used for extraction of the essential oil from the leaves. The same compounds, in similar amounts, were identified by both methods, confirming the MD-SPME method is highly reliable. Compared with SD, MD-SPME required less time (only 2 min), less sample (1.0 g), and no organic solvent. These results are indicative of the suitability of MD-SPME-GC-MS for simple, rapid, and solvent-free analysis of plant essential oils.
Hao Ye, Jie Ji, Chunhui Deng, Ning Yao, Ning Li, Xiangmin Zhang
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