The development of generic inhibitors in order to control the formation of amyloid fibrils and early oligomers is still an unmet medical need. Here, we demonstrate the applicability of electrochemical analysis for the detection of β-sheet breaker peptide ligands that act as excellent inhibitors of Alzheimer's disease (AD) amyloid-β (Aβ) fibrils and oligomers in vitro. As the case study, a well-defined β-sheet breaker pentapeptide (LPFFD, FibIII) was utilized with Aβ1-42 peptides. Square wave voltammetry (SWV) measurements were confirmed with simultaneous fluorescence analysis of the same incubated Aβ samples using a well-known fluorescent marker of β-sheet formation, Thioflavin T (ThT). Significant changes in the electrochemical signals were observed for the interaction of the Aβ oligomers with FibIII at the early stages of aggregation. The electrochemical approach, in principle, allowed monitoring β-sheet breaker-Aβ interactions on the time scale of aggregation in a label-free and cost-effective format using screen-printed carbon strip (SPCS) electrodes.
Publication date: July 2013Source:Journal of Solid State Chemistry, Volume 203 Author(s): S. Kumar , K. Singh , M. Miclau , Ch. Simon , C. Martin , A. Maignan The change from antiferromagnetism induced ferroelectricity to spin glass ferroelectric relaxor has been studied along the ... more
Publication date: July 2013Source:Journal of Solid State Chemistry, Volume 203 Author(s): Brian B. Kitchen , Nina Verdal , Terrence J. Udovic , John J. Rush , Michael R. Hartman , Daniel J. DeVries To investigate the previously reported low-temperature phase transition in rubidium ... more
Publication date: July 2013Source:Journal of Solid State Chemistry, Volume 203 Author(s): T. Sasikala , L. Rama Moorthy , A. Mohan Babu , T. Srinivasa Rao The present work reports the absorption, photoluminescence and decay properties of singly doped Dy3+ and co-doped Dy3+/Tm3+ ions ... more
Elsevier announced the acquisition through its subsidiary MDL Information Systems GmbH, of the Beilstein Database, the world's largest compilation of chemical facts and the premier database in the field of organic chemistry.
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