The interaction of sulfated polysaccharides (fucoidans) from brown seaweeds Laminaria japonica, Laminaria cichorioides, and Fucus evanescens with Toll-like receptors (TLRs) expressed on membranes of human embryonic kidney epithelial cells (HEK293-null, HEK293-TLR2/CD14, HEK293-hTLR4/CD14-MD2 and HEK293-hTLR2/6), has been investigated. In vitro fucoidans specifically interacted with TLR-2, TLR-4, and the heterodimer TLR-2/6; this resulted in activation of the transcription nuclear factor NF-κB. Composition the hydrolyzed fucoidan from F. evanescens was analyzed by gas-liquid chromatography and chromatography-mass spectrometry. Results indicated the absence of 3-hydroxytetradecanoic acid (3-OHC14), the basic fatty acid component of lipopolysaccharides in the analyzed preparation. Thus, the obtained results suggest that fucoidans from brown seaweeds possessing immunotropic activity are independent ligands for TLRs. They can induce genetically determined biochemical processes of protection organisms against pathogenic microorganisms.
I. D. Makarenkova, D. Yu. Logunov, A. I. Tukhvatulin, I. B. Semenova, T. N. Zvyagintseva, V. I. Gorbach, S. P. Ermakova, N. N. Besednova
Formation and protective properties of conversion coatings on 1370 aluminum alloy that were obtained in an IFKhANAL-3 alkaline conversion solution are studied. The subsequent filling of the coatings in an inhibitor solution is shown to produce coatings that are resistant to pitting corrosio ... more
It is shown that carbon nanomaterials (ultradispersed diamond-graphite batch mixture, ultradispersed diamonds) introduced into basic electrolytes cause significant intensification of the process of micro-plasma formation of ceramic coatings on aluminum alloys of different compositions, incl ... more
Corrosion-electrochemical behavior of composite layers formed by high-speed laser sintering of nanoscale Fe-Ni powders (3.2 and 10 wt % Ni) is studied with the use of anodic potentiodynamic curves recorded in a borate buffer solution in a neutral environment. Composite layers produced are s ... more