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Nonglycosylated erythropoietin bearing acetamidomethyl protecting groups at the cysteine residues has been synthesized via chemical methods. Alanine ligation was used to assemble four peptide fragments, themselves prepared by solid phase peptide synthesis. This work outlines a route for the synthesis of homogeneous glycosylated erythropoietin.
| Authors: | John A. Brailsford; Samuel J. Danishefsky | |
| Journal: | Proceedings of the National Academy of Sciences current issue | |
| Volume: | 109 | |
| Issue: | 19 | |
| Year: | 2012 | |
| Pages: | 7196 | |
| DOI: | 10.1073/pnas.1202762109 | |
| Publication date: | 08-05-2012 |
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