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The excitation and detection of high-order multiple quantum coherences among 13C nuclear spins are demonstrated in the samples of [1-13C]-l-alanine and 13C labeled amyloid fibrils at a spinning frequency of 20 kHz. The technique is based on the double-quantum average Hamiltonian prepared by the DRAMA-XY4 pulse sequence. Empirically, we find that multiple supercycles are required to suppress the higher-order effects for real applications. Measurements for the fibril samples formed by the polypeptides of PrP(113–127) provide the first solid-state NMR evidence for the stacking of multiple β-sheet layers at the structural core of amyloid fibrils
| Authors: | Fang-Chieh Chou; Tim W. T. Tsai; Hsin-Mei Cheng; Jerry C. C. Chan | |
| Journal: | Journal of Physical Chemistry B | |
| Year: | 2012 | |
| DOI: | 10.1021/jp303455w | |
| Publication date: | 08-06-2012 |
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