Efficient selection and enumeration of low-abundance biological cells are highly important in a variety of applications. For example, the clinical utility of circulating tumor cells (CTCs) in peripheral blood is recognized as a viable biomarker for the management of various cancers, in which the clinically relevant number of CTCs per 7.5 ml of blood is two to five. Although there are several methods for isolating rare cells from a variety of heterogeneous samples, such as immunomagnetic-assisted cell sorting and fluorescence-activated cell sorting, they are fraught with challenges. Microsystem-based technologies are providing new opportunities for selecting and isolating rare cells from complex, heterogeneous samples. Such approaches involve reductions in target-cell loss, process automation, and minimization of contamination issues. In this review, we introduce different application areas requiring rare cell analysis, conventional techniques for their selection, and finally microsystem approaches for low...
Udara Dharmasiri; Małgorzata A. Witek; Andre A. Adams; Steven A. Soper
We review some outstanding puzzles and experimental anomalies in hadron physics that appear to challenge conventional wisdom and, in some cases, the foundations of quantum chromodynamics (QCD). We also discuss possible solutions and propose new tests and experiments that could illuminate th ... more
We review the methods developed for combining the parton shower approximation to quantum chromodynamics with fixed-order perturbation theory so as to achieve next-to-leading-order (NLO) accuracy for inclusive observables. These developments have made it possible to generate fully simulated ... more
General classes of mechanisms for generating small neutrino masses are surveyed from a top-down (superstring) perspective. In particular, string constructions have motivated various possibilities involving higher-dimensional operators, string instantons, and wave-function overlaps in large ... more