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Development and validation of an HPLC-FLD method for rapid determination of histamine in skipjack tuna fish (Katsuwonus pelamis)

This study compared and validated two methods of high-performance liquid chromatography: fluorescence detection (HPLC-FLD) and UV-vis detection. Recoveries greater than 55% at all spiking levels using UV-vis detection could not be obtained. However, derivatization with pre-column o-phthalaldehyde (OPA) was found to efficiently enhance the method sensitivity. The analytical method was validated based on the following criteria: linearity, sensitivity, accuracy, precision, repeatability, and recovery. For fluorescence detection, excellent linear correlation with R2=0.9977 was observed over the range of 5.0 to 100.0 mg/L. The minimum detection limit was calculated to be 1.5 mg/kg, while the limit of quantification (LOQ) value of the...

Authors:   Saeed Tahmouzi, Didehban , Ramin, Khaksar , Mehran, Ghasemlou
Journal:   Food Chemistry
Year:   2010
DOI:   10.1016/j.foodchem.2010.11.060
Publication date:   17-Nov-2010
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