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1,582 Newest Publications in acta crystallographica section f

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Neutron and high‐resolution room‐temperature X‐ray data collection from crystallized lytic polysaccharide monooxygenase

03-Nov-2015 | John‐Paul Bacik, Sophanit Mekasha, Zarah Forsberg, Andrey Kovalevsky, Jay C. Nix, Matthew J. Cuneo, Leighton Coates, ..., Acta Crystallographica Section F, 2015

Bacteria and fungi express lytic polysaccharide monooxgyenase (LPMO) enzymes that act in conjunction with canonical hydrolytic sugar‐processing enzymes to rapidly convert polysaccharides such as chitin, cellulose and starch to single monosaccharide products. In order to gain a better ...

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Expression and crystallographic studies of the ligand‐binding region of the human endocytic collagen receptor uPARAP

03-Nov-2015 | Cai Yuan, Joy He Huang, Min Liu, Mingdong Huang, Acta Crystallographica Section F, 2015

Urokinase plasminogen activator receptor‐associated protein (uPARAP) is an endocytic receptor that internalizes collagen for lysosomal degradation and plays an important role in matrix remodelling. Previous recombinant protein production of uPARAP in Pichia pastoris generated protein with highly ...

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Cloning, expression, purification, characterization, crystallization and X‐ray crystallographic analysis of recombinant Der f 21 (rDer f 21) from Dermatophagoides farinae

03-Nov-2015 | Sze Lei Pang, Kok Lian Ho, Jitka Waterman, Aik‐Hong Teh, Fook Tim Chew, Chyan Leong Ng, Acta Crystallographica Section F, 2015

Dermatophagoides farinae is one of the major house dust mite (HDM) species that cause allergic diseases. N‐terminally His‐tagged recombinant Der f 21 (rDer f 21), a group 21 allergen, with the signal peptide truncated was successfully overexpressed in an Escherichia coli expression system. The ...

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Structure of a fungal form of aspartate semialdehyde dehydrogenase from Cryptococcus neoformans

03-Nov-2015 | Gopal Dahal, Ronald E. Viola, Acta Crystallographica Section F, 2015

Aspartate semialdehyde dehydrogenase (ASADH) functions at a critical junction in the aspartate‐biosynthetic pathway and represents a valid target for antimicrobial drug design. This enzyme catalyzes the NADPH‐dependent reductive dephosphorylation of β‐aspartyl phosphate to produce the key ...

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Crystallization and biochemical characterization of the human spliceosomal Aar2–Prp8RNaseH complex

03-Nov-2015 | Karine Santos, Marco Preussner, Anna Christina Heroven, Gert Weber, Acta Crystallographica Section F, 2015

In eukaryotes, the removal of nuclear noncoding sequences (pre‐mRNA splicing) is catalyzed by the spliceosome, which consists of five ribonucleoprotein particles (U1, U2, U4, U5 and U6 snRNPs, each with a respective snRNA) and a plethora of protein factors that aid spliceosomal maturation, ...

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Crystallization and crystallographic analysis of an Arabidopsis nuclear proteinaceous RNase P

03-Nov-2015 | Franziska Pinker, Philippe Giegé, Claude Sauter, Acta Crystallographica Section F, 2015

RNase P activity is ubiquitous and involves the 5′ maturation of precursor tRNAs. For a long time, it was thought that all RNases P were ribonucleoproteic enzymes. However, the characterization of RNase P in human mitochondria and in plants revealed a novel kind of RNase P composed of protein ...

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Crystallization and X‐ray diffraction analysis of the HMG domain of the chondrogenesis master regulator Sox9 in complex with a ChIP‐Seq‐identified DNA element

03-Nov-2015 | Saravanan Vivekanandan, Balasubramanian Moovarkumudalvan, Julien Lescar, Prasanna R. Kolatkar, Acta Crystallographica Section F, 2015

Sox9 is a fundamental sex‐determining gene and the master regulator of chondrogenesis, and is involved in the development of various vital organs such as testes, kidney, heart and brain, and in skeletal development. Similar to other known Sox transcription factors, Sox9 recognizes and binds DNA ...

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Structure of Escherichia coli tryptophanase purified from an alkaline‐stressed bacterial culture

03-Nov-2015 | Stephane Rety, Patrick Deschamps, Nicolas Leulliot, Acta Crystallographica Section F, 2015

Tryptophanase is a bacterial enzyme involved in the degradation of tryptophan to indole, pyruvate and ammonia, which are compounds that are essential for bacterial survival. Tryptophanase is often overexpressed in stressed cultures. Large amounts of endogenous tryptophanase were purified from ...

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Production, crystallization and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2

03-Nov-2015 | Saara Laulumaa, Matthew P. Blakeley, Arne Raasakka, Martine Moulin, Michael Härtlein, Petri Kursula, Acta Crystallographica Section F, 2015

The molecular details of the formation of the myelin sheath, a multilayered membrane in the nervous system, are to a large extent unknown. P2 is a peripheral membrane protein from peripheral nervous system myelin, which is believed to play a role in this process. X‐ray crystallographic studies ...

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Structure of the HIV‐1 reverse transcriptase Q151M mutant: insights into the inhibitor resistance of HIV‐1 reverse transcriptase and the structure of the nucleotide‐binding pocket of Hepatitis B virus polymerase

03-Nov-2015 | Akiyoshi Nakamura, Noriko Tamura, Yoshiaki Yasutake, Acta Crystallographica Section F, 2015

Hepatitis B virus polymerase (HBV Pol) is an important target for anti‐HBV drug development; however, its low solubility and stability in vitro has hindered detailed structural studies. Certain nucleotide reverse transcriptase (RT) inhibitors (NRTIs) such as tenofovir and lamivudine can inhibit ...

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