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Design Concepts in Absorbance Optical Systems for Analytical Ultracentrifugation

03-Jul-2018 | Joseph Pearson; Marcel Hofstetter; Thomas Dekorsy; Michael Totzeck; Helmut Coelfen, Analyst, 2018

Analytical ultracentrifugation is a powerful technique for analyzing particles in solution, and has proved valuable for a wide range of applications in chemistry, biochemistry and material sciences for many years. The field is presently seeing a resurgence of instrument development from ...

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A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control

03-Jul-2018 | Yangyang Jiang; Lin Du; Yuanming Li; Quanquan Mu; Zhongxu Cui; Jia Zhou; Wenming Wu, Analyst, 2018

Unlike conventional approaches that require bulky and expensive pumping equipment, herein, we present a simple method for self-activated microdroplet generation and transport inside a long microchannel. The high gas-pressure in the syringes is used to provide the built-in power of self-priming so ...

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Correction: Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation

03-Jul-2018 | Jiulong Su; Jinhua Dong; Tetsuya Kitaguchi; Yuki Ohmuro-Matsuyama; Hiroshi Ueda, Analyst, 2018

Correction for ‘Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation’ by Jiulong Su et al., Analyst, 2018, 143, 2096–2101.

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Distance–based Paper Sensor for Determination of Chloride ion Using Silver Nanoparticles

03-Jul-2018 | Kamonchanok Phoonsawat; Nalin Ratnarathorn; Charles Henry; Wijitar Dungchai, Analyst, 2018

We report the first development of a distance-based paper sensor for a simple, inexpensive, instrument-free, and portable determination of chloride ions. Our analysis reaction is based on the oxidative etching of silver nanoparticles (AgNPs) to form AgCl in the presence of Cl⁻ and H2O2. The H2O2 ...

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Towards Mapping Electrostatic Interactions between Kdo2-Lipid A and Cationic Antimicrobial Peptides via Ultraviolet Photodissociation Mass Spectrometry

03-Jul-2018 | Christopher M Crittenden; Lindsay Jane Morrison; Mignon D Fitzpatrick; Allison P Myers; Elisa T Novelli; Jake Rosenb ..., Analyst, 2018

Cationic antimicrobial peptides (CAMPs) have known to act as multi-modal weapons against Gram-negative bacteria. As a new approach to investigate the nature of the interactions between CAMPs and the surfaces of bacteria, native mass spectrometry and two MS/MS strategies (ultraviolet ...

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A ratiometric fluorescent hydrogen peroxide chemosensor manipulated by ICT-activated FRET mechanism and its bioimaging application in living cells and zebrafish

03-Jul-2018 | Kaixin Xu; Longwei He; Xueling Yang; Yunzhen Yang; Weiying Lin, Analyst, 2018

A H2O2-responsive fluorescence chemosensor (CNBE) with ratiometric emission signal was elaborately designed and synthesized. The ratio signal of chemosensor was manipulated by interplaying ICT-activated FRET mechanism. The ratiometric fluorescent imaging was successfully applied to detect H2O2 ...

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A light-up fluorescence assay for tumor cell detection based on bifunctional split aptamers

03-Jul-2018 | Yuqiong Sun; Baoyin Yuan; Meitao Deng; Qing Wang; Jin Huang; Qiuping Guo; Jianbo Liu; Xiaohai Yang; Kemin Wang, Analyst, 2018

The light-up aptamers have attracted growing attention due to their advantages of label-free and low fluorescence background. In this work, we developed a light-up fluorescence assay for label-free detection of tumor cells based on the bifunctional split aptamer (BFSA) which contained two DNA ...

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Microfluidic Single-Cell Analysis of Oxidative Stress in Dictyostelium discoideum

27-Jun-2018 | Kathy Rodogiannis; Jessica T Duong; Michelle Kovarik, Analyst, 2018

Microfluidic chemical cytometry is a powerful technique for examining chemical contents of individual cells, but applications have focused on cells from multicellular organisms, especially mammals. We demonstrate the first use of microfluidic chemical cytometry to examine a unicellular organism, ...

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SABRE hyperpolarization enables high-sensitivity 1H and 13C benchtop NMR spectroscopy

27-Jun-2018 | Peter M. Richardson; Andrew J. Parrott; Olga Semenova; Alison Nordon; Simon B. Duckett; Meghan E. Halse, Analyst, 2018

Benchtop NMR spectrometers operating with low magnetic fields of 1–2 T at sub-ppm resolution show great promise as analytical platforms that can be used outside the traditional laboratory environment for industrial process monitoring. One current limitation that reduces the uptake of benchtop NMR ...

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Predictive modelling of water contact angle of surfaces using Attenuated Total Reflection – Fourier Transform Infrared (ATR-FTIR) chemical imaging and Partial Least Squares Regression (PLSR)

26-Jun-2018 | Sindhuraj Mukherjee; JOSE Ángel MARTINEZ-GONZALEZ; Denis P Dowling; Aoife A. Gowen, Analyst, 2018

The static water contact angle (CA) quantifies the degree of wetting that occurs when a surface encounters a liquid, e.g. water. This property is a result of factors such as surface chemistry, local roughness and is an important analytical parameter linked to the suitability of a surface for a ...

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