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86 Newest Publications in bmc biochemistry
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Identification of Avian Wax Synthases
04-02-2012 | Eva-Maria Biester, Janine Hellenbrand, Jens Gruber, Mats Hamberg, Margrit Frentzen, BMC Biochemistry, 2012
Background: Bird species show a high degree of variation in the composition of their preen gland waxes. For instance, galliform birds like chicken contain fatty acid esters of 2,3-alkanediols, while Anseriformes like goose or Strigiformes like barn owl contain wax monoesters in their preen ...
Enzymatic activity and immunoreactivity of Aca s 4, an alpha-amylase allergen from the storage mite Acarus siro
31-01-2012 | Jana Pytelkova, Martin Lepsik, Miloslav Sanda, Pavel Talacko, Lucie Maresova, Michael Mares, BMC Biochemistry, 2012
Background: Enzymatic allergens of storage mites that contaminate stored food products are poorly characterized. We describe biochemical and immunological properties of the native alpha-amylase allergen Aca s 4 from Acarus siro, a medically important storage mite. Results: A. siro produced a ...
Hyaluronan synthase mediates dye translocation across liposomal membranes
25-01-2012 | Andria P Medina, Jialing Lin, Paul H Weigel, BMC Biochemistry, 2012
Background: Hyaluronan (HA) is made at the plasma membrane and secreted into the extracellular medium or matrix by phospolipid-dependent hyaluronan synthase (HAS), which is active as a monomer. Since the mechanism by which HA is translocated across membranes is still unresolved, we assessed ...
Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I
16-01-2012 | Mohammad A Hossain, Sereyrath Ngeth, Teddy Chan, Michael N Oda, Gordon A Francis, BMC Biochemistry, 2012
Background: ATP-binding cassette transporter A1 (ABCA1) mediates the lipidation of exchangeable apolipoproteins, the rate-limiting step in the formation of high density lipoproteins (HDL). We previously demonstrated that HDL oxidized ex vivo by peroxidase-generated tyrosyl radical ...
Topological characterisation and identification of critical domains within glucosyltransferase IV (GtrIV) of Shigella flexneri
22-12-2011 | Anesh Nair, Haralambos Korres, Naresh K Verma, BMC Biochemistry, 2011
Background: The three bacteriophage genes gtrA, gtrB and gtr(type) are responsible for O-antigen glucosylation in Shigella flexneri. Both gtrA and gtrB have been demonstrated to be highly conserved and interchangeable among serotypes while gtr(type) was found to be specific to each serotype, ...
Low affinity PEGylated hemoglobin from Trematomus bernacchii, a model for hemoglobin-based blood substitutes
20-12-2011 | Daniela Coppola, Stefano Bruno, Luca Ronda, Cristiano Viappiani, Stefania Abbruzzetti, Guido di Prisco, Cinzia Verde ..., BMC Biochemistry, 2011
Background: Conjugation of human and animal hemoglobins with polyethylene glycol has been widely explored as a means to develop blood substitutes, a novel pharmaceutical class to be used in surgery or emergency medicine. However, PEGylation of human hemoglobin led to products with ...
Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds
01-09-2011 | Rosa Lonneborg; Peter Brzezinski, BMC Biochemistry, 2011
Background: The transcriptional regulators DntR, NagR and NtdR have a high sequence identity and belong to the large family of LysR type transcriptional regulators (LTTRs). These three regulators are all involved in regulation of genes identified in pathways for degradation of aromatic ...
Application of Celluspots peptide arrays for the analysis of the binding specificity of epigenetic reading domains to modified histone tails
31-08-2011 | Ina Bock; Srikanth Kudithipudi; Raluca Tamas; Goran Kungulovski; Arunkumar Dhayalan; Albert Jeltsch, BMC Biochemistry, 2011
Background: Epigenetic reading domains are involved in the regulation of gene expression and chromatin state by interacting with histones in a post-translational modification specific manner. A detailed knowledge of the target modifications of reading domains, including enhancing and ...
Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence
24-08-2011 | Ireneusz Sobolewski; Katarzyna Polska; Agnieszka Zylicz-Stachula; Joanna Jezewska-Frackowiak; Janusz Rak; Piotr Skowron, BMC Biochemistry, 2011
Background: Restriction endonucleases are widely applied in recombinant DNA technology. Among them, enzymes of class IIS, which cleave DNA beyond recognition sites, are especially useful. We use BsaI enzyme for the pinpoint introduction of halogen nucleobases into DNA. This has been done for ...
Gallus gallus NEU3 sialidase as model to study protein evolution mechanism based on rapid evolving loops.
23-08-2011 | Edoardo Giacopuzzi; Sergio Barlati; Augusto Preti; Bruno Venerando; Eugenio Monti; Giuseppe Borsani; Roberto Bresciani, BMC Biochemistry, 2011
Background: Large surface loops contained within compact protein structures and not involved in catalytic process have been proposed as preferred regions for protein family evolution. These loops are subjected to lower sequence constraints and can evolve rapidly in novel structural variants. ...
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