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336 Newest Publications in proceedings of the national academy of sciences current issue

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miRNA regulation of BK polyomavirus replication during early infection [Microbiology]

14-05-2013 | Nicole M. Broekema; Michael J. Imperiale, Proceedings of the National Academy of Sciences current issue, 2013

Viral microRNAs (miRNAs) play an important role during infection by posttranscriptionally regulating both host and viral gene expression. However, the function of many viral miRNAs remains poorly understood. In this study, we investigated the role of the BK polyomavirus (BKPyV) miRNA in ...

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Bats are a major natural reservoir for hepaciviruses and pegiviruses [Microbiology]

14-05-2013 | Phenix-Lan Quan; Cadhla Firth; Juliette M. Conte; Simon H. Williams; Carlos M. Zambrana-Torrelio; Simon J. Anthony; ..., Proceedings of the National Academy of Sciences current issue, 2013

Although there are over 1,150 bat species worldwide, the diversity of viruses harbored by bats has only recently come into focus as a result of expanded wildlife surveillance. Such surveys are of importance in determining the potential for novel viruses to emerge in humans, and for optimal ...

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Conserved glycolipid termini in capsular polysaccharides synthesized by ATP-binding cassette transporter-dependent pathways in Gram-negative pathogens [Microbiology]

07-05-2013 | Lisa M. Willis; Jacek Stupak; Michele R. Richards; Todd L. Lowary; Jianjun Li; Chris Whitfield, Proceedings of the National Academy of Sciences current issue, 2013

Bacterial capsules are surface layers made of long-chain polysaccharides. They are anchored to the outer membrane of many Gram-negative bacteria, including pathogens such as Escherichia coli, Neisseria meningitidis, Haemophilus influenzae, and Pasteurella multocida. Capsules protect pathogens ...

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Controlling electron transfer at the microbe-mineral interface [Microbiology]

07-05-2013 | David J. Richardson; Julea N. Butt; Thomas A. Clarke, Proceedings of the National Academy of Sciences current issue, 2013

Remarkably, many bacteria live and thrive in the earth’s subsurface by respiring extracellular insoluble minerals. Okamato et al. (1) in PNAS report how this process may be accelerated by the presence of flavins that bind as cofactors to electron transport proteins on the cell surface that ...

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The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus OF4 [Microbiology]

07-05-2013 | Laura Preiss; Adriana L. Klyszejko; David B. Hicks; Jun Liu; Oliver J. Fackelmayer; Özkan Yildiz; Terry A. Krulwich; ..., Proceedings of the National Academy of Sciences current issue, 2013

The c-rings of ATP synthases consist of individual c-subunits, all of which harbor a conserved motif of repetitive glycine residues (GxGxGxG) important for tight transmembrane α-helix packing. The c-ring stoichiometry determines the number of ions transferred during enzyme operation and has a ...

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Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquinones [Microbiology]

07-05-2013 | Akihiro Okamoto; Kazuhito Hashimoto; Kenneth H. Nealson; Ryuhei Nakamura, Proceedings of the National Academy of Sciences current issue, 2013

Extracellular redox-active compounds, flavins and other quinones, have been hypothesized to play a major role in the delivery of electrons from cellular metabolic systems to extracellular insoluble substrates by a diffusion-based shuttling two-electron-transfer mechanism. Here we show that ...

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HPV virions hitchhike a ride on retromer complexes [Microbiology]

30-04-2013 | Martin J. Sapp, Proceedings of the National Academy of Sciences current issue, 2013

Nonenveloped viruses use cellular pathways to gain access to and traverse the cytoplasm of host cells. Indeed, many of the by now well-established pathways have been initially identified during the study of virus entry. In PNAS, Lipovsky et al. add to the growing list of transport pathways ...

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Differential protein partitioning within the herpesvirus tegument and envelope underlies a complex and variable virion architecture [Microbiology]

23-04-2013 | Kevin Patrick Bohannon; Yonggun Jun; Steven P. Gross; Gregory Allan Smith, Proceedings of the National Academy of Sciences current issue, 2013

The herpesvirus virion is a multilayered structure consisting of a DNA-filled capsid, tegument, and envelope. Detailed reconstructions of the capsid are possible based on its icosahedral symmetry, but the surrounding tegument and envelope layers lack regular architecture. To circumvent ...

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Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution [Microbiology]

23-04-2013 | Boris R. Belitsky; Abraham L. Sonenshein, Proceedings of the National Academy of Sciences current issue, 2013

The CodY protein is a global transcriptional regulator that controls, directly or indirectly, expression of more than 100 genes and operons in Bacillus subtilis. We used in vitro DNA affinity purification combined with massively parallel sequencing, to identify B. subtilis chromosomal DNA ...

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Flagella stator homologs function as motors for myxobacterial gliding motility by moving in helical trajectories [Microbiology]

16-04-2013 | Beiyan Nan; Jigar N. Bandaria; Amirpasha Moghtaderi; Im-Hong Sun; Ahmet Yildiz; David R. Zusman, Proceedings of the National Academy of Sciences current issue, 2013

Many bacterial species use gliding motility in natural habitats because external flagella function poorly on hard surfaces. However, the mechanism(s) of gliding remain elusive because surface motility structures are not apparent. Here, we characterized the dynamics of the Myxococcus xanthus ...

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