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1,382 Newest Publications in proceedings of the national academy of sciences current issue

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Arabidopsis OR proteins are the major posttranscriptional regulators of phytoene synthase in controlling carotenoid biosynthesis [Plant Biology]

17-Mar-2015 | Xiangjun Zhou; Ralf Welsch; Yong Yang; Daniel Álvarez; Matthias Riediger; Hui Yuan; Tara Fish; Jiping Liu; Theodore ..., Proceedings of the National Academy of Sciences current issue, 2015

Carotenoids are indispensable natural pigments to plants and humans. Phytoene synthase (PSY), the rate-limiting enzyme in the carotenoid biosynthetic pathway, and ORANGE (OR), a regulator of chromoplast differentiation and enhancer of carotenoid biosynthesis, represent two key proteins that ...

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Quorum-sensing Salmonella selectively trigger protein expression within tumors [Engineering]

17-Mar-2015 | Charles A. Swofford; Nele Van Dessel; Neil S. Forbes, Proceedings of the National Academy of Sciences current issue, 2015

Salmonella that secrete anticancer proteins have the potential to eliminate tumors, but nonspecific expression causes damage to healthy tissue. We hypothesize that Salmonella, integrated with a density-dependent switch, would only express proteins in tightly packed colonies within tumors. To test ...

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Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution [Biochemistry]

17-Mar-2015 | Matthias Hillenbrand; Christian Schori; Jendrik Schöppe; Andreas Plückthun, Proceedings of the National Academy of Sciences current issue, 2015

Agonist binding to G-protein–coupled receptors (GPCRs) triggers signal transduction cascades involving heterotrimeric G proteins as key players. A major obstacle for drug design is the limited knowledge of conformational changes upon agonist binding, the details of interaction with the different ...

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Dual role of tree florigen activation complex component FD in photoperiodic growth control and adaptive response pathways [Plant Biology]

10-Mar-2015 | Szymon Tylewicz; Hiroyuki Tsuji; Pál Miskolczi; Anna Petterle; Abdul Azeez; Kristoffer Jonsson; Ko Shimamoto; Rishik ..., Proceedings of the National Academy of Sciences current issue, 2015

A complex consisting of evolutionarily conserved FD, FLOWERING LOCUS T (FT) proteins is a regulator of floral transition. Intriguingly, FT orthologs are also implicated in developmental transitions distinct from flowering, such as photoperiodic control of bulbing in onions, potato tuberization, ...

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Rapid labeling of intracellular His-tagged proteins in living cells [Cell Biology]

10-Mar-2015 | Yau-Tsz Lai; Yuen-Yan Chang; Ligang Hu; Ya Yang; Ailun Chao; Zhi-Yan Du; Julian A. Tanner; Mee-Len Chye; Chengmin Qi ..., Proceedings of the National Academy of Sciences current issue, 2015

Small molecule-based fluorescent probes have been used for real-time visualization of live cells and tracking of various cellular events with minimal perturbation on the cells being investigated. Given the wide utility of the (histidine)6-Ni2+-nitrilotriacetate (Ni-NTA) system in protein ...

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Tonoplast CBL-CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis [Plant Biology]

10-Mar-2015 | Ren-Jie Tang; Fu-Geng Zhao; Veder J. Garcia; Thomas J. Kleist; Lei Yang; Hong-Xia Zhang; Sheng Luan, Proceedings of the National Academy of Sciences current issue, 2015

Although Mg2+ is essential for a myriad of cellular processes, high levels of Mg2+ in the environment, such as those found in serpentine soils, become toxic to plants. In this study, we identified two calcineurin B-like (CBL) proteins, CBL2 and CBL3, as key regulators for plant growth under ...

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Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis [Biochemistry]

03-Mar-2015 | Janette Kropat; Sean D. Gallaher; Eugen I. Urzica; Stacie S. Nakamoto; Daniela Strenkert; Stephen Tottey; Andrew Z. ..., Proceedings of the National Academy of Sciences current issue, 2015

Inorganic elements, although required only in trace amounts, permit life and primary productivity because of their functions in catalysis. Every organism has a minimal requirement of each metal based on the intracellular abundance of proteins that use inorganic cofactors, but elemental sparing ...

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G{alpha}i/o-coupled receptor signaling restricts pancreatic {beta}-cell expansion [Physiology]

03-Mar-2015 | Miles Berger; David W. Scheel; Hector Macias; Takeshi Miyatsuka; Hail Kim; Phuong Hoang; Greg M. Ku; Gerard Honig; A ..., Proceedings of the National Academy of Sciences current issue, 2015

Gi-GPCRs, G protein-coupled receptors that signal via Gα proteins of the i/o class (Gαi/o), acutely regulate cellular behaviors widely in mammalian tissues, but their impact on the development and growth of these tissues is less clear. For example, Gi-GPCRs acutely regulate insulin release from ...

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Cell-specific proteomic analysis in Caenorhabditis elegans [Biochemistry]

03-Mar-2015 | Kai P. Yuet; Meenakshi K. Doma; John T. Ngo; Michael J. Sweredoski; Robert L. J. Graham; Annie Moradian; Sonja Hess; ..., Proceedings of the National Academy of Sciences current issue, 2015

Proteomic analysis of rare cells in heterogeneous environments presents difficult challenges. Systematic methods are needed to enrich, identify, and quantify proteins expressed in specific cells in complex biological systems including multicellular plants and animals. Here, we have engineered a ...

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Regulation and aggregation of intrinsically disordered peptides [Biophysics and Computational Biology]

03-Mar-2015 | Zachary A. Levine; Luca Larini; Nichole E. LaPointe; Stuart C. Feinstein; Joan-Emma Shea, Proceedings of the National Academy of Sciences current issue, 2015

Intrinsically disordered proteins (IDPs) are a unique class of proteins that have no stable native structure, a feature that allows them to adopt a wide variety of extended and compact conformations that facilitate a large number of vital physiological functions. One of the most well-known IDPs ...

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