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32 Newest Publications of de Gruyter
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Serum mesothelin and megakaryocyte potentiating factor in pancreatic and biliary cancers
13-12-2011 | Elad Sharon; Jingli Zhang; Kevin Hollevoet; Seth M. Steinberg; Ira Pastan; Masanori Onda; Jochen Gaedcke; B. ..., Clinical Chemistry , 2011
Abstract Background: Tumor mesothelin overexpression is present in different malignancies, including the majority of patients with pancreatic or biliary cancers. The objective of this study was to evaluate the use of shed serum mesothelin and megakaryocyte potentiating factor (MPF) ...
Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA
13-12-2011 | Johan Frederik Berg Arendt; Edward V. Quadros; Ebba Nexo, Clinical Chemistry , 2011
Abstract Background: The cell-surface receptor, TCblR/CD320, binds and internalizes transcobalamin-bound cobalamin. So far, no assay has been developed in order to quantify the soluble form of the receptor. Methods: This study developed an ELISA employing a monoclonal antibody that recognized ...
Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis
16-09-2011 | Ciprian N. Silaghi; Dana Fodor; Victor Cristea; Alexandra M. Crăciun, Clinical Chemistry , 2011
Abstract Background: Matrix Gla-protein (MGP) is a calcification inhibitor produced by cartilage and vessel walls. Arthritis is defined by inflammation, cartilage and bone destruction/formation and articular calcifications. Our objectives were to evaluate ucMGP levels in synovial fluid (SF) ...
Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum
23-08-2011 | Evi Debruyne; Marijn Speeckaert; Yannick Vande Weygaerde; Joris Delanghe, Clinical Chemistry , 2011
Abstract Background: Gc-globulin is a polymorphic protein with three phenotypes: Gc 1-1, Gc 2-1 and Gc 2-2. Deglycosylation of Gc-globulin results in a Gc-macrophage activating factor (Gc-MAF). This study investigated the potential of MAF as a tumour marker and the influence of Gc-phenotypes ...
Trefoil factor family peptides are increased in the saliva of children with mucositis
28-07-2011 | Fran Verey; Ebba Nexo; Rosemary Greenwood; Monica Berry; Anthony P. Corfield, Clinical Chemistry , 2011
Abstract Background: Mucositis is a painful ulcerative condition of the oral cavity and gastrointestinal tract, occurring in association with chemotherapy and radiotherapy regimes. Trefoil factor family peptides (TFF, trefoil peptides), present in saliva, contribute to epithelial restitution ...
Development of a microwell adapted immunoblot system with recombinant antigens for distinguishing human herpesvirus (HHV)6A and HHV6B and detection of human cytomegalovirus
14-07-2011 | Andrea Thäder-Voigt; Enno Jacobs; Werner Lehmann; Dirk Bandt, Clinical Chemistry , 2011
Abstract Background: The human cytomegalovirus (HCMV) and the human herpesvirus 6 (HHV6) are widely distributed in the human population. The variants A and B of HHV6 are closely related to each other and cannot be distinguished by common serological methods like enzyme-linked immunosorbent ...
Bayesian analysis of an international ELISA comparability study
05-07-2011 | Katy Klauenberg; Bernd Ebert; Jan Voigt; Monika Walzel; James E. Noble; Alex E. Knight; Clemens Elster, Clinical Chemistry , 2011
Abstract Background: Immunoassays are biochemical tests applied to measure even very small amounts of substance using the highly specific binding between an antibody and its antigen. They have a wide range of applications. The measurement however, might be associated with substantial ...
Significant elevation of plasma pentraxin 3 in patients with pelvic inflammatory disease
17-06-2011 | Chih-Chia Chang; Po-Hui Wang; Pen-Hua Su; Ding-Bang Lin; Tsung-Ho Ying; Shun-Fa Yang; Yi-Hsien Hsieh, Clinical Chemistry , 2011
Abstract Background: Pentraxin 3 (PTX3) plays an important role in innate immune responses and in inflammation disease. The aim of this study was to investigate the diagnostic and prognostic potential of PTX3 in pelvic inflammatory disease (PID) and correlate it with the severity and outcome ...
Analytical performance of a multiplexed, bead-based cytokine detection system in small volume samples
10-06-2011 | Martin Bomert; Gabi Köllisch; Marjut Roponen; Roger Lauener; Harald Renz; Petra I. Pfefferle; Nadia Al-F ..., Clinical Chemistry , 2011
Abstract Background: Multiplexed cytokine measurement offers many advantages over the conventional enzyme-linked immunosorbent assay (ELISA) format when applied in large-scale epidemiological studies or clinical trials. In the present study we set out to define the reliability and consistency ...
Modified phosphatidylserine-dependent antithrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies
08-06-2011 | Polona Žigon; Aleš Ambrožič; Saša Čučnik; Tanja Kveder; Blaž Rozman; Bo ..., Clinical Chemistry , 2011
Abstract Background: Two approaches for detecting anti-prothrombin antibodies have been described. The first detects antibodies against prothrombin alone and the second, phos-phatidylserine-dependent antiprothrombin antibodies. The latter more often correlate with clinical manifestations of ...
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