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42 Newest Publications of de Gruyter

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Comparison of different collection procedures and two methods for DNA isolation from saliva

13-12-2011 | Jaroslava Durdiaková; Natália Kamodyová; Daniela Ostatníková; Barbora Vlková; Pete ..., Clinical Chemistry , 2011

Abstract Background: The non-invasive, flexible and easy sample collection makes saliva an interesting source of DNA for research and diagnostic purposes. The aim of our study was to find the most suitable collection method for biological material from the oral cavity and the most effective DNA ...

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The evaluation of colloidal gold immunochromatographic assay (GICA) for rapid diagnosis of influenza A disease

13-09-2011 | Xuehui Li; Hangwei Chen; Juan Wei; Nan Lv; Lanhua You, Clinical Chemistry , 2011

Abstract Background: A low cost colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A virus was developed. The assay was evaluated in this study. Methods: Six hundred and twenty-six patients were enrolled. All patients contributed two pharyngeal swabs, one used for ...

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Rapid HLA-B27 screening with real-time TaqMan PCR: a clinical validation in the Dutch population

06-09-2011 | Elianne A. Roelandse-Koop; Bert Buisman; Erik J. van Hannen; Anneke van der Zee; Wouter Kortlandt; Mirjam H.A. ..., Clinical Chemistry , 2011

Abstract Background: Human leukocyte antigen B27 (HLA-B27) is strongly associated with ankylosing spondylitis. The B27 allele is present in 90% of patients with this disease, whereas it is present in only 9% of Caucasians. Molecular detection of HLA-B27 is traditionally based on allele specific ...

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Association of a functional polymorphism in the MMP7 gene promoter with susceptibility to vulnerable carotid plaque in a Han Chinese Population

04-07-2011 | Xiao-Fei Hu; Xiao-Ping Jin; Pei-Yang Hu; Min Zhu; Feng Wang; Xian-Fang Lin; Wei-Ling Li; Hong Ni; Li-Hua Yang, Clinical Chemistry , 2011

Abstract Background: Matrix metalloproteinase-7 (MMP-7) may play an important role in the development of vulnerable carotid plaque. An A-to-G transition (–181A/G) in the promoter region of MMP7 is functional in vitro by altering the transcriptional activity of the gene. The aim of this study was ...

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Genotyping of the hemochromatosis HFE p.H63D and p.C282Y mutations by high-resolution melting with the Rotor-Gene 6000® instrument

17-06-2011 | Paulo Caleb Junior Lima Santos; Renata Alonso Gadi Soares; Jose Eduardo Krieger; Elvira Maria Guerra-Shinohara; ..., Clinical Chemistry , 2011

Abstract Background: The genotyping of HFE p.C282Y and p.H63D mutations is one of the most requested molecular analyses in the laboratorial routine. In this scenario, the main aim was to develop a genotyping assay that has advantages compared to other methods. Methods: Genotypes for the HFE ...

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Differential expression of CDC25 phosphatases splice variants in human breast cancer cells

16-06-2011 | Hélène Albert; Susana Santos; Eric Battaglia; Miguel Brito; Carolino Monteiro; Denyse Bagrel, Clinical Chemistry , 2011

Abstract Background: CDC25 phosphatases control cell cycle progression by activating cyclin dependent kinases. The three CDC25 isoforms encoding genes are submitted to alternative splicing events which generate at least two variants for CDC25A and five for both CDC25B and CDC25C. An ...

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Prospective study of first stroke in relation to plasma homocysteine and MTHFR 677C>T and 1298A>C genotypes and haplotypes – evidence for an association with hemorrhagic stroke

02-06-2011 | Xuehui Li; Hangwei Chen; Juan Wei; Nan Lv; Lanhua You, Clinical Chemistry , 2011

Abstract Background: A low cost colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A virus was developed. The assay was evaluated in this study. Methods: Six hundred and twenty-six patients were enrolled. All patients contributed two pharyngeal swabs, one used for ...

more

High resolution melting analysis to genotype the most common variants in the HFE gene

01-06-2011 | Roberta V. Marotta; Olivia Turri; Antonella Morandi; Manuela Murano; Gianlodovico Melzi d’Eril; Maria Lui ..., Clinical Chemistry , 2011

Abstract Background: High resolution melting (HRM) analysis of PCR amplicons was recently introduced as a closed-tube, rapid, and inexpensive method of genotyping. This study evaluated this system as an option for detecting the three most common mutations in the HFE gene (C282Y, H63D, S65C), ...

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Expression and role of the cell surface protease seprase/fibroblast activation protein-α (FAP-α) in astroglial tumors

23-05-2011 | Rolf Mentlein; Kirsten Hattermann; Charles Hemion; Achim A. Jungbluth; Janka Held-Feindt, Biological Chemistry, 2011

Abstract Seprase or fibroblast activation protein-α (FAP-α) is a cell-surface serine protease that was previously described nearly exclusively on reactive and tumor stromal fibroblasts and thought to be involved in tissue remodeling. We investigated the expression and significance of FAP-α in ...

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Identification of lily pollen 14-3-3 isoforms and their subcellular and time-dependent expression profile

23-05-2011 | Heidi Pertl; Simon Rittmann; Waltraud X. Schulze; Gerhard Obermeyer, Biological Chemistry, 2011

Abstract 14-3-3 proteins are major regulators in plant development and physiology including primary metabolism and signal transduction pathways, typically via a phosphorylation-dependent interaction with a target protein. Four full-length 14-3-3 isoforms were identified in pollen grains of Lilium ...

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