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425 Newest Publications of Portland Press

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Comparisons of subunit 5A and 5B isoenzymes of yeast cytochrome c oxidase

15-12-2014 | Dodia R; Meunier B; Kay CWM; Rich PR, Biochemical Journal, 2014

Subunit 5 of Saccharomyces cerevisiae cytochrome c oxidase (CcO) is essential for assembly and has two isoforms, 5A and 5B. 5A is expressed under normoxic conditions, whereas 5B is expressed at very low oxygen tensions. As a consequence, COX5A-deleted strains (Δcox5A) have no or only low levels ...

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A context-independent N-glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation

15-12-2014 | Huttner S; Veit C; Vavra U; Schoberer J; Dicker M; Maresch D; Altmann F; Strasser R, Biochemical Journal, 2014

N-glycosylation of proteins plays an important role in the determination of the fate of newly synthesized glycoproteins in the endoplasmic reticulum (ER). Specific oligosaccharide structures recruit molecular chaperones that promote folding or mannose-binding lectins that assist in the clearance ...

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TREHALOSE-6-PHOSPHATE SYNTHASE 1 is not the only active TPS in {Less than}i{Greater than}Arabidopsis thaliana.{Less than}/i{Greater than}

12-12-2014 | Delorge I; Figueroa C; Feil R; Lunn J; Van Dijck P, Biochemical Journal, 2014

Trehalose metabolism is essential for normal growth and development in higher plants. It is synthesized in a two-step pathway catalyzed by trehalose-6-phosphate (Tre6P) synthase (TPS) and trehalose phosphatase. Arabidopsis thaliana has 11 TPS or TPS-like proteins, which belong to two distinct ...

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TARP {gamma}-8 glycosylation regulates the surface expression of AMPA receptors

11-12-2014 | Zheng C; Chang K; Suh Y; Roche K, Biochemical Journal, 2014

Transmembrane AMPA receptor regulatory protein (TARP) γ-8 is an auxiliary subunit of AMPA receptors that is widely distributed in the hippocampus. It has been shown that TARP γ-8 promotes surface expression of AMPA receptors; however, how TARP γ-8 regulates the expression of AMPA receptors ...

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Expression of different bacterial cytotoxins is controlled by two global transcription factors, CRP and Fis, that cooperate in a shared-recruitment mechanism.

08-12-2014 | Rossiter A; Godfrey R; Connolly J; Busby S; Henderson I; Browning D, Biochemical Journal, 2014

Pet is a cytotoxic autotransporter protein secreted by the pathogenic Enteroaggregative Escherichia coli strain 042. Expression of Pet is co-dependent on two global transcription regulators: CRP (cyclic AMP receptor protein) and Fis (factor for inversion stimulation). At the pet promoter CRP ...

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Raptor ablation in skeletal muscle decreases Cav1.1 expression and affects the function of the excitation-contraction coupling supramolecular complex

28-11-2014 | Lopez R; Mosca B; Treves S; Maj M; Bergamelli L; Calderon J; Bentzinger C; Romanino K; Hall M; R{u}egg M; Delbono O; ..., Biochemical Journal, 2014

The protein mammalian target of rapamycin (mTOR) is a serine/threonine kinase regulating a number of biochemical pathways controlling cell growth. mTOR exists in two complexes termed mTORC1 and mTORC2. Raptor is associated with mTORC1 and is essential for its function. Ablation of Raptor in ...

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MiR-98 delays skeletal muscle differentiation by downregulating E2F5

25-11-2014 | Kropp J; Degerny C; Morozova N; Pontis J; Harel-bellan A; Polesskaya A, Biochemical Journal, 2014

A genome-wide screen had previously shown that knocking down miR-98 and let-7g, two miRNAs of the let-7 family, leads to a dramatic increase of terminal myogenic differentiation. Here we report that a transcriptomic analysis of human myoblasts where miR-98 was knocked down revealed that ...

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Inositol pyrophosphates regulate RNA polymerase I-mediated rRNA transcription in {Less than}i{Greater than}Saccharomyces cerevisiae{Less than}/i{Greater than}

24-11-2014 | Thota S; Unnikannan C; Thampatty S; Manorama R; Bhandari R, Biochemical Journal, 2014

Ribosome biogenesis is an essential cellular process regulated by the metabolic state of a cell. We examined whether inositol pyrophosphates, energy-rich derivatives of inositol which act as metabolic messengers, play a role in ribosome synthesis in the budding yeast, Saccharomyces cerevisiae. ...

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Steatosis inhibits liver cell store-operated Ca{Less than}sup{Greater than}2{+}{Less than}/sup{Greater than} entry and reduces ER Ca{Less than}sup{Greater than}2{+}{Less than}/sup{Greater than} through a protein kinase C dependent mechanism

24-11-2014 | Wilson C; Ali E; Scrimgeour N; Martin A; Hua J; Tallis G; Rychkov G; Barritt G, Biochemical Journal, 2014

Lipid accumulation in hepatocytes can lead to non-alcoholic fatty liver disease, which can progress to non-alcoholic steatohepatitis and type 2 diabetes. Hormone-initiated release of Ca2+ from the endoplasmic reticulum stores and subsequent replenishment of these stores by Ca2+ entry through ...

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A quantitative proteomics-based signature of platinum sensitivity in ovarian cancer cell lines

19-11-2014 | Fan G; Wrzeszczynski K; Fu C; Su G; Pappin D; Lucito R; Tonks N, Biochemical Journal, 2014

Although DNA encodes the molecular instructions that underlie control of cell function, it is the proteins that are primarily responsible for implementing those instructions. Therefore, quantitative analyses of the proteome would be expected to yield insights into important candidates for the ...

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