07-05-2013 | Wang C; Lo H; Lin S; Chen H, Biochemical Journal, 2013

GCM1 is a short-lived transcription factor essential for placental development. The F-box protein, FBW2, which contains five tryptophan-aspartate (WD) repeats, recognizes GCM1 and mediates its ubiquitination via the SCFFBW2 E3 ligase complex. Although the interaction between GCM1 and FBW2 is ...

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07-05-2013 | Shankarling G; Lynch K, Biochemical Journal, 2013

Understanding functional distinctions between related splicing regulatory proteins is critical to deciphering tissue-specific control of alternative splicing. The hnRNP L and hnRNP LL proteins are paralogues that have overlapping but distinct expression patterns and functional consequences. ...

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01-05-2013 | Han K; Ayer D, Biochemical Journal, 2013

The MondoA:Mlx transcription complex plays a pivotal role in glucose homeostasis by activating target gene expression in response to glucose-6-phosphate (G6P), the first reaction intermediate in glycolysis. Thioredoxin-interacting protein (TXNIP) is a direct and glucose-responsive target of ...

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15-04-2013 | Bett JS; Ibrahim AFM; Garg AK; Kelly V; Pedrioli P; Rocha S; Hay RT, Biochemical Journal, 2013

HIF1A (hypoxia-inducible factor 1α) is the master regulator of the cellular response to hypoxia and is implicated in cancer progression. Whereas the regulation of HIF1A protein in response to oxygen is well characterized, less is known about the fate of HIF1A mRNA. In the present study, we ...

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10-04-2013 | Scudieri P; Sondo E; Caci E; Ravazzolo R; Galietta L, Biochemical Journal, 2013

TMEM16A and TMEM16B proteins are Ca2+ activated Cl- channels (CaCCs) with eight putative transmembrane segments. As shown previously, expression of TMEM16B generates CaCCs characterized by a ten-fold lower Ca2+ affinity and by faster activation and deactivation kinetics with respect to ...

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08-04-2013 | D'Antonio C; Molinski S; Ahmadi S; Huan L; Wellhauser L; Bear C, Biochemical Journal, 2013

Mutations in the CLCN5 gene cause Dent’s disease because they reduce the functional expression of the ClC-5 chloride: proton transporter in recycling endosomes of proximal tubule epithelial cells. The majority (60%) of these disease-causing mutations in ClC-5 are misprocessed and retained in ...

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05-04-2013 | Kim W; Hsiao J; Bhatia S; Glaros E; Don A; Tsuruoka S; Shannon Weickert C; Halliday G, Biochemical Journal, 2013

Members of ATP-binding cassette subfamily A (ABCA) are characterized by their ability to transport lipids across cellular membranes and regulate lipid homeostasis in the brain and peripheral tissues. ABCA8 is a little-known member of this subfamily, originally cloned from human brain ...

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05-04-2013 | Davies P; Hinkle K; Sukar N; Sepulveda B; Mesias R; Serrano G; Alessi D; Beach T; Benson D; White III C; Cowell R; D ..., Biochemical Journal, 2013

Missense mutations in leucine-rich repeat kinase 2 (LRRK2) are a major cause of Parkinson’s disease (PD). Several antibodies against LRRK2 have been developed, but results using these polyclonal antibodies have varied widely leading to conflicting conclusions. To address this challenge, The ...

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04-04-2013 | Gantke T; Boussouf S; Janzen J; Morrice N; Howell S; M{u}hlberger E; Ley S, Biochemical Journal, 2013

Activation of Protein Kinase R (PKR) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK-293 cells. Co-expression with Ebola virus VP35, which blocked plasmid activation of PKR, substantially increased production of ...

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03-04-2013 | Azevedo M; Sanders P; Krejany E; Nie C; Fu P; Bach L; Wunderlich G; Crabb B; Gilson P, Biochemical Journal, 2013

Plasmodium falciparum calcium-dependent protein kinase 1 (PfCDPK1) is highly expressed in parasite asexual blood and mosquito stages. Its role is still poorly understood, but unsuccessful gene knockout attempts suggest it is essential for schizont development and/or RBC invasion. Here, by ...

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