05-04-2013 | Song L; Bowen C; Gelmann E, Biochemical Journal, 2013

NKX3.1 is a prostate tumor suppressor protein that binds to topoisomerase I and enhances its DNA cleavage activity. We show that the NKX3.1 homeodomain binds to a region of topoisomerase I spanning the junction between core and linker domains. NKX3.1 activated N-terminal truncated ...

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01-04-2013 | Ju J-h; Yang W; Oh S; Nam K; Lee K-m; Noh D-y; Shin I, Biochemical Journal, 2013

In breast cancer, the HER2 (human epidermal growth factor receptor 2) receptor tyrosine kinase is associated with extremely poor prognosis and survival. Notch signalling has a key role in cell-fate decisions, especially in cancer-initiating cells. The Notch intracellular domain produced by ...

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18-03-2013 | Bentley C; Jurinka S; Kljavin N; Vartanian S; Ramani S; Gonzalez L; Yu K; Modrusan Z; Du P; Bourgon R; Neve R; Stokoe D, Biochemical Journal, 2013

The mutant forms of K, N and HRas are known to drive the initiation and progression of a number of human cancers, but less is known about the role of wild-type (WT) Ras alleles and isoforms in this process. We used zinc finger nucleases targeting HRas and NRas to modify both alleles of these ...

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13-03-2013 | Li S; Ran Y; Zhang D; Chen J; Zhu D, Biochemical Journal, 2013

Unbalanced apoptosis is a major cause of structural remodeling of vasculatures associated with pulmonary arterial hypertension (PAH), while the underlying mechanisms are still elusive. MicroRNAs (miRNAs) regulate the expression of several proteins that are important for the cell fate ...

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07-03-2013 | Encarnacao P; Ramirez V; Zhang C; Aneskievich B, Biochemical Journal, 2013

TNFα-induced protein 3-interacting protein 1 (TNIP1) is a corepressor of RAR and PPAR. Additionally, it can reduce signaling stemming from cell membrane receptors such as those for TNFα and EGF. Consequently, it influences a variety of receptor-mediated events as diverse as transcription, ...

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01-03-2013 | Li B; Pi Z; liu L; Zhang B; Huang X; Hu P; Chevet E; Yi P; Liu J, Biochemical Journal, 2013

Induction of Endoplasmic Reticulum (ER) stress-mediated apoptosis in cancer cells represents an alternative approach for cancer therapy. Whether fibroblast growth factor 2 (FGF-2)-induced survival signals may interact with ER stress signalling in cancer cells remains elusive. In this work, we ...

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01-03-2013 | Zencir S; Sike A; Dobson MJ; Ayaydin F; Boros I; Topcu Z, Biochemical Journal, 2013

ADA (alteration/deficiency in activation) 3 is a conserved component of several transcriptional adaptor and HAT (histone acetyltransferase) complexes that regulate RNA polymerase II-mediated gene expression. Within the HAT complexes ADA3 is associated with ADA2 and the HAT GCN5 (general ...

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15-02-2013 | Coothankandaswamy V; Elangovan S; Singh N; Prasad PD; Thangaraju M; Ganapathy V, Biochemical Journal, 2013

SLC5A8 (solute carrier gene family 5A, member 8) is a sodium-coupled transporter for monocarboxylates. Among its substrates are the HDAC (histone deacetylase) inhibitors butyrate, propionate and pyruvate. Expression of SLC5A8 is silenced in cancers via DNA methylation, and ectopic expression ...

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14-02-2013 | Lenglet G; Depauw S; Mendy D; David-Cordonnier M, Biochemical Journal, 2013

In the view to develop new DNA alkylating antitumor drugs, evaluating the precise mechanism of action and the molecular/cellular consequences of the alkylation is of point of major interest. The benzo-b-acronycine derivative S23906-1 alkylates guanines in the minor groove of the DNA helix and ...

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07-02-2013 | Kim S; Ao Z; Warnock G; McIntosh C, Biochemical Journal, 2013

The incretins, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are gastrointestinal hormones conferring a number of beneficial effects on β-cell secretion, survival and proliferation. In an earlier study, it was demonstrated that delayed rectifier ...

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