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140 Newest Publications of Portland Press

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Ubiquitin-C-terminal hydrolases cleave isopeptide and peptide-linked ubiquitin from structured proteins but do not edit ubiquitin homopolymers

09-Dec-2014 | Bett J; Ritorto M; Ewan R; Jaffray E; Virdee S; Chin J; Knebel A; Kurz T; Trost M; Tatham M; Hay R, Biochemical Journal, 2014

Modification of proteins with ubiquitin (Ub) occurs through a variety of topologically distinct Ub linkages, including Ube2W-mediated monoubiquitylation of N-terminal alpha amines to generate peptide-linked linear mono-Ub fusions. Protein ubiquitylation can be reversed by the action of ...


Kinetic Characterization of a Cocaine Hydrolase Engineered from Mouse Butyrylcholinesterase

08-Dec-2014 | Chen X; Huang X; Geng L; Xue L; Hou S; Zheng X; Brimijoin S; Zheng F; Zhan C, Biochemical Journal, 2014

Mouse butyrylcholinesterase (mBChE) and an mBChE-based cocaine hydrolase (mCocH, i.e. the A199S/S227A/S287G/A328W/Y332G mutant) have been characterized for their catalytic activities against cocaine, i.e. naturally occurring (-)-cocaine, in comparison with the corresponding human BChE (hBChE) and ...


The disease-associated mutation of the mitochondrial sulphydryl oxidase Erv1 impairs cofactor binding during its catalytic reaction

01-Oct-2014 | Ceh-Pavia E; Ang S; Spiller M; Lu H, Biochemical Journal, 2014

Erv1(essential for respiration and viability 1) is an FAD-dependent sulphydryl oxidase of the Erv/ALR (augmenter of liver regeneration) sub-family. It is an essential component of the mitochondrial import and assembly (MIA) pathway, playing an important role in the oxidative folding of ...


Chromatin and oxygen sensing in the context of JmjC histone demethylases

15-Sep-2014 | Shmakova A; Batie M; Druker J; Rocha S, Biochemical Journal, 2014

Responding appropriately to changes in oxygen availability is essential for multicellular organism survival. Molecularly, cells have evolved intricate gene expression programmes to handle this stressful condition. Although it is appreciated that gene expression is co-ordinated by changes in ...


Mutation of tryptophan 93 of MauG to tyrosine causes loss of bound Ca{Less than}sup{Greater than}2{+}{Less than}/sup{Greater than} and alters the kinetic mechanism of tryptophan tryptophylquinone cofactor biosynthesis

11-Sep-2013 | Shin S; Feng M; Davidson V, Biochemical Journal, 2013

The diheme enzyme MauG catalyzes a six-electron oxidation required for posttranslational modification of a precursor of methylamine dehydrogenase (preMADH) to complete the biosynthesis of its tryptophan tryptophylquinone (TTQ) cofactor. Trp93 of MauG is positioned midway between its two hemes, ...


On the catalytic mechanism of dimeric dUTPases

04-Sep-2013 | Hemsworth G; Gonz{a}lez-Pacanowska D; Wilson K, Biochemical Journal, 2013

The Tritryps Trypanosoma brucei, T. cruzi and Leishmania donovani are responsible for great morbidity and mortality in developing countries. Their dimeric dUTPases are members of the all-a NTP pyrophosphohydrolase family and represent promising drug targets due to their essential nature and ...


Completing the folate biosynthesis pathway in {Less than}i{Greater than}Plasmodium falciparum{Less than}/i{Greater than}: {Less than}i{Greater than}p{Less than}/i{Greater than}-aminobenzoate is produced by a highly divergent, promiscuous aminodeoxychorismate lyase

19-Aug-2013 | Magnani G; Lomazzi M; Peracchi A, Biochemical Journal, 2013

Enzymes that produce or recycle folates are the targets of widely used antimalarial drugs. Despite the interest in the folate metabolism in Plasmodium falciparum, the molecular identification of aminodeoxychorismate lyase (ADCL), which synthesizes the p-aminobenzoate moiety of folate, remained ...


A novel fluorescent assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases

06-Aug-2013 | Maz{a}{n} M; Blanco N; Kov{a}{c with caron}ov{a} K; Zemkov{a} Z; {R}ehulka P; Farka{s with caron} V; Arroyo J, Biochemical Journal, 2013

Mechanical properties of fungal cell walls are largely determined by composition and mutual cross-linking of their macromolecular components. Previous work showed that the Crh proteins are required for the formation of cross-links between chitin and glucan at the Saccharomyces cerevisiae cell ...


Crystal structures of SCP2-thiolases of Trypanosomatidae, human pathogens causing widespread tropical diseases: the importance for catalysis of the cysteine of the unique HDCF loop

02-Aug-2013 | Harijan R; Kiema T; Karjalainen M; Janardan N; Murthy M; Weiss M; Michels P; Wierenga R, Biochemical Journal, 2013

Thiolases are essential CoA dependent enzymes in lipid metabolism. Here we report on crystal structures of trypanosomal and leishmanial SCP2-thiolases. Trypanosomatidae cause various widespread, devastating (sub)-tropical diseases, for which adequate treatment is lacking. The structures reveal ...


A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism

09-Jul-2013 | Goyer A; Hasnain G; Frelin O; Ralat M; Gregory J; Hanson A, Biochemical Journal, 2013

Genes specifying the thiamin monophosphate phosphatase and adenylated thiazole diphosphatase steps in fungal and plant thiamin biosynthesis remain unknown, as do genes for thiamin diphosphate (ThDP) hydrolysis in thiamin metabolism. A distinctive Nudix domain fused to thiamin diphosphokinase ...


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