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124 Newest Publications of Portland Press

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Structural analysis of the di-adenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3{'}-dATP

27-May-2015 | M{u}ller M; Deimling T; Hopfner K; Witte G, Biochemical Journal, 2015

The identification of the essential bacterial second messenger cyclic-di-AMP synthesized by the DNA-integrity scanning protein DisA opened up a new and emerging field in bacterial signaling. To further analyze the di-adenylate cyclase reaction catalyzed by the DAC domains of DisA, we crystallized ...

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Design of novel fluorescent mitochondria-targeted peptides with iron-selective sensing activity

26-May-2015 | Abbate V; Reelfs O; Hider R; Pourzand C, Biochemical Journal, 2015

Mitochondrial labile iron (LI) plays a crucial role in oxidative injuries and pathologies. At present, there is no organelle-specific sensitive iron sensor which can reside exclusively in the mitochondria and reliably monitor levels of LI in this organelle. Here we describe the development of ...

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The purification and characterization of ATP synthase complexes from the mitochondria of four fungal species

15-May-2015 | Liu S; Charlesworth TJ; Bason JV; Montgomery MG; Harbour ME; Fearnley IM; Walker JE, Biochemical Journal, 2015

The ATP synthases have been isolated by affinity chromatography from the mitochondria of the fungal species Yarrowia lipolytica, Pichia pastoris, Pichia angusta and Saccharomyces cerevisiae. The subunit compositions of the purified enzyme complexes depended on the detergent used to solubilize and ...

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Erythropoietin mediated expression of Placenta Growth Factor is regulated via activation of hypoxia inducible factor -1{alpha} and post-transcriptionally by mir214 in Sickle Cell Disease

16-Apr-2015 | Gonsalves C; Li C; Mwa Mpollo M; Pullarkat V; Malik P; Tahara S; Kalra V, Biochemical Journal, 2015

Placental growth factor (PlGF) plays an important role in various pathological conditions and diseases such as inflammation, cancer, atherosclerosis and sickle cell disease (SCD). Abnormally high PlGF levels in SCD patients are associated with increased inflammation and pulmonary hypertension and ...

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{Less than}i{Greater than}In vitro{Less than}/i{Greater than} reconstitution of cotranslational D1 insertion reveals a role of the cpSec/Alb3 translocase and Vipp1 in photosystem II biogenesis

24-Mar-2015 | Walter B; Hristou A; Nowaczyk M; Sch{u}nemann D, Biochemical Journal, 2015

Photosystem II (PS II) is a multi subunit complex localized in the thylakoid membrane that performs the light dependent photosynthetic charge separation. The PS II reaction center comprises, amongst others, the D1 protein. De novo synthesis and repair of PS II require efficient mechanisms for ...

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The purification and characterization of ATP synthase complexes from the mitochondria of four fungal species

11-Mar-2015 | Liu S; Charlesworth T; Bason J; Montgomery M; Harbour M; Fearnley I; Walker J, Biochemical Journal, 2015

The ATP synthases have been isolated by affinity chromatography from the mitochondria of the fungal species, Yarrowia histolytica, Pichia pastoris, Pichia angusta and Saccharomyces cerevisae. The subunit compositions of the purified enzyme complexes depended upon the detergent used to solubilise ...

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Inositol pyrophosphates regulate RNA polymerase I-mediated rRNA transcription in Saccharomyces cerevisiae

15-Feb-2015 | Thota SG; Unnikannan CP; Thampatty SR; Manorama R; Bhandari R, Biochemical Journal, 2015

Ribosome biogenesis is an essential cellular process regulated by the metabolic state of a cell. We examined whether inositol pyrophosphates, energy-rich derivatives of inositol that act as metabolic messengers, play a role in ribosome synthesis in the budding yeast, Saccharomyces cerevisiae. ...

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Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase

04-Dec-2014 | Hou X; Wu W; Duan X; Liu N; Li H; Fu J; Dou S; Li M; Xi X, Biochemical Journal, 2014

Recent advances in G-quadruplex (G4) studies have confirmed that G4 structures exist in living cells and may have detrimental effects on various DNA transactions. How helicases resolve G4, however, has just begun to be studied and remains largely unknown. Here we use single-molecule fluorescence ...

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{Less than}i{Greater than}Bombyx mori{Less than}/i{Greater than} prothoracicostatic peptide receptor is allosterically activated via a G{alpha}{Less than}sub{Greater than}i/o{Less than}/sub{Greater than} protein-biased signaling cascade by {Less than}i{Greater than}Drosophila{Less than}/i{Greater than} sex peptide

25-Nov-2014 | He X; Zang J; Yang H; Huang H; Shi Y; Zhu C; Zhou N, Biochemical Journal, 2014

In insects, molting and metamorphosis are strictly regulated by ecdysteroids. Ecdysteroid synthesis is positively or negatively controlled by several neuropeptides. The prothoracicostatic peptide BmPTSP, isolated from the larval brain of Bombyx mori, has been demonstrated to inhibit ecdysteroid ...

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Inositol pyrophosphates regulate RNA polymerase I-mediated rRNA transcription in {Less than}i{Greater than}Saccharomyces cerevisiae{Less than}/i{Greater than}

24-Nov-2014 | Thota S; Unnikannan C; Thampatty S; Manorama R; Bhandari R, Biochemical Journal, 2014

Ribosome biogenesis is an essential cellular process regulated by the metabolic state of a cell. We examined whether inositol pyrophosphates, energy-rich derivatives of inositol which act as metabolic messengers, play a role in ribosome synthesis in the budding yeast, Saccharomyces cerevisiae. ...

more

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