01-07-2013 | Dudek KM; Suter L; Darras VM; Marczylo EL; Gant TW, Biochemical Journal, 2013

Recent work has demonstrated the importance of post-transcriptional gene regulation in toxic responses. In the present study, we used two rat models to investigate mRNA translation in the liver following xenobiotic-induced toxicity. By combining polysome profiling with genomic methodologies, ...

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01-07-2013 | Andrews PGP; He Z; Tzenov YR; Popadiuk C; Kao KR, Biochemical Journal, 2013

Increased protein synthesis during cell proliferation is accompanied by a compensatory increase in efficient ribosome production, but the mechanisms by which cells adapt to this requirement are not fully understood. In the present study, we demonstrate evidence that Pygo (Pygopus), a protein ...

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01-07-2013 | Kim HE; Bae E; Jeong D-y; Kim M-J; Jin W-J; Park S-W; Han G-S; Carman GM; Koh E; Kim K-S, Biochemical Journal, 2013

PPARγ (peroxisome-proliferator-activated receptor γ) is a master transcription factor involved in adipogenesis through regulating adipocyte-specific gene expression. Recently, lipin1 was found to act as a key factor for adipocyte maturation and maintenance by modulating the C/EBPα ...

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07-06-2013 | Singh K; Saleh A; Bhadra A; Roy I, Biochemical Journal, 2013

Maintenance of cellular redox homeostasis forms an important part of the cellular defence mechanism and continued cell viability. Despite extensive studies, the role of the chaperone Hsp104 in propagation of misfolded protein aggregates in the cell and generation of oxidative stress remains ...

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01-06-2013 | Lane DJR; Huang ML-H; Richardson DR, Biochemical Journal, 2013

Does the hormone of iron metabolism, hepcidin, exhibit 'self-control'? Hepcidin is a small, disulfide-rich peptide synthesized by the liver, which plays a keystone role in regulating systemic iron metabolism in mammals. Hepcidin acts by binding and triggering the lysosomal degradation of the ...

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17-05-2013 | Swalm B; Hallenbeck K; Majer C; Jin L; Scott M; Moyer M; Copeland R; Wigle T, Biochemical Journal, 2013

Histone H3 lysine 27 (H3K27) methylation is an important epigenetic modification that regulates gene transcription. In humans, EZH1 and EZH2 are the only enzymes capable of catalyzing methylation of H3K27. There is great interest in understanding structure-function relationships for EZH2, as ...

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14-05-2013 | Hyv{o}nen M; Koponen T; Weisell J; Pietil{a} M; Khomutov A; Veps{a}l{a}inen J; Alhonen L; Kein{a}nen T, Biochemical Journal, 2013

We previously showed that the polyamine spermidine is indispensable for differentiation of 3T3-L1 preadipocytes. Here, we studied the mechanism of spermidine function by using polyamine biosynthesis inhibitor α-difluoromethylornithine in combination with the metabolically stable polyamine ...

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07-05-2013 | Wang C; Lo H; Lin S; Chen H, Biochemical Journal, 2013

GCM1 is a short-lived transcription factor essential for placental development. The F-box protein, FBW2, which contains five tryptophan-aspartate (WD) repeats, recognizes GCM1 and mediates its ubiquitination via the SCFFBW2 E3 ligase complex. Although the interaction between GCM1 and FBW2 is ...

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01-05-2013 | Han K; Ayer D, Biochemical Journal, 2013

The MondoA:Mlx transcription complex plays a pivotal role in glucose homeostasis by activating target gene expression in response to glucose-6-phosphate (G6P), the first reaction intermediate in glycolysis. Thioredoxin-interacting protein (TXNIP) is a direct and glucose-responsive target of ...

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30-04-2013 | Kim H; Bae E; Jeong D; Kim M; Jin W; Park S; Han G; Carman G; Koh E; Kim K, Biochemical Journal, 2013

Peroxisome proliferator-activated receptor-γ (PPARγ) is a master transcription factor involved in adipogenesis through regulating adipocyte-specific gene expression. Recently, lipin1 is known to act as a key factor for the adipocyte maturation and maintenance with modulating C/EBPα and PPARγ ...

more