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3,345 Newest Publications about the topic microscopes


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Multispectral optical imaging combined in situ with XPS or ToFSIMS and principal component analysis

20-May-2016 | Peter J. Cumpson, Ian W. Fletcher, Richard Burnett, Naoko Sano, Anders J. Barlow, Jose F. Portoles, Lisa W. Li, Andr ..., Surface and Interface Analysis, 2016

All X‐ray photoelectron spectroscopy (XPS) and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) instruments have optical cameras to image the specimen under analysis, and often to image the sample holder as it enters the system too. These cameras help the user find the appropriate ...


High‐Resolution Vertical Observation of Intracellular Structure Using Magnetically Responsive Microplates

17-May-2016 | Tetsuhiko Teshima, Hiroaki Onoe, Soichiro Tottori, Hiroka Aonuma, Takeomi Mizutani, Koki Kamiya, Hirotaka Ishihara, ..., Small, 2016

A vertical confocal observation system capable of high‐resolution observation of intracellular structure is demonstrated. The system consists of magnet‐active microplates to rotate, incline, and translate single adherent cells in the applied magnetic field. Appended to conventional confocal ...


Combining gradient ascent search and support vector machines for effective autofocus of a field emission–scanning electron microscope

09-May-2016 | S. DEMBÉLÉ, O. LEHMANN, K. MEDJAHER, N. MARTURI, N. PIAT, Journal of Microscopy, 2016

Summary Autofocus is an important issue in electron microscopy, particularly at high magnification. It consists in searching for sharp image of a specimen, that is corresponding to the peak of focus. The paper presents a machine learning solution to this issue. From seven focus measures, ...


General morphology and ultrastructure of the female reproductive apparatus of Trichomalopsis shirakii crawford (Hymenoptera, Pteromalidae)

06-May-2016 | Ning Mao, Pu Tang, Hong‐Wei Tian, Min Shi, Xue‐Xin Chen, Microscopy Research and Technique, 2016

ABSTRACT The morphology and ultrastructure of the female reproductive system were examined for a larval–pupal parasitoid Trichomalopsis shirakii Crawford of Oulema oryzae Kuwayama using light and electron microscopes. The reproductive system includes two ovaries, two pairs of accessory glands, ...


Data Analysis for Total Internal Reflection Fluorescence Microscopy

01-May-2016 | Asbury, C. L., CSH Protocols, 2016

In the microscopes we use to analyze total internal reflection fluorescence (TIRF), the emitted fluorescence is split chromatically, using dichroic filters, into either two or three different colors ("channels"). In our two-color instrument, the green emission wavelengths (405–488 nm; for imaging ...


Cuticle and subsurface ornamentation of intact plant leaf epidermis under confocal and superresolution microscopy

25-Apr-2016 | Michael A. Urban, Richard S. Barclay, Mayandi Sivaguru, Surangi W. Punyasena, Microscopy Research and Technique, 2016

ABSTRACT Plant cuticle micromorphology is an invaluable tool in modern ecology and paleoecology. It has expanded our knowledge of systematic relationships among diverse plant groups and can be used to identify fossil plants. Furthermore, fossil plant leaf micromorphology is used for ...


Antisolvent crystallization and solid‐state polymerization of bisphenol‐A polycarbonate

31-Mar-2016 | Xuesong Chang, Tongmei Ding, Hengshui Tian, Tiantian Wang, Journal of Applied Polymer Science, 2016

ABSTRACT Bisphenol‐A polycarbonate (BAPC) was synthesized by solid‐state polymerization (SSP) using a semicrystalline prepolymer crystallized by antisolvent method. The antisolvent crystallization was investigated as a function of antisolvent types using X‐ray diffraction (XRD), different ...


The cutting of ultrathin sections with the thickness less than 20 nm from biological specimens embedded in resin blocks

31-Mar-2016 | Jana Nebesářová, Pavel Hozák, Luděk Frank, Petr Štěpan, Marie Vancová, Microscopy Research and Technique, 2016

ABSTRACT Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the ...


Light sheet microscopy and live imaging of plants

28-Mar-2016 | BÉATRICE BERTHET, ALEXIS MAIZEL, Journal of Microscopy, 2016

Summary Light sheet fluorescence microscopy (LSFM) is increasingly used to investigate biological processes in animals as well as in plants. LSFM achieves optical sectioning by the selective illumination of a single plane of the sample with a sheet of laser light while simultaneously recording ...


Micro‐CT scouting for transmission electron microscopy of human tissue specimens

08-Feb-2016 | A.G. MORALES, E.S. STEMPINSKI, X. XIAO, A. PATEL, A. PANNA, K.N. OLIVIER, P.J. MCSHANE, C. ROBINSON, A.J. GEORGE, D. ..., Journal of Microscopy, 2016

Summary Transmission electron microscopy (TEM) provides sub‐nanometre‐scale details in volumetric samples. Samples such as pathology tissue specimens are often stained with a metal element to enhance contrast, which makes them opaque to optical microscopes. As a result, it can be a lengthy ...


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