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3,308 Newest Publications about the topic microscopes

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Cryo-Electron Microscopy and the Amazing Race to Atomic Resolution

14-May-2015 | Elad Binshtein; Melanie D. Ohi, Biochemistry, 2015

Cryo-electron microscopy (cryo-EM), the structural analysis of samples embedded in vitreous ice, is a powerful approach for determining three-dimensional (3D) structures of biological specimens. Over the past two decades, this technique has been used to successfully calculate subnanometer ...

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Sample holder for axial rotation of specimens in 3D microscopy

06-May-2015 | T. BRUNS, S. SCHICKINGER, H. SCHNECKENBURGER, Journal of Microscopy, 2015

Summary In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three‐dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an ...

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Adaptive optics in spinning disk microscopy: improved contrast and brightness by a simple and fast method

04-May-2015 | V. FRAISIER, G. CLOUVEL, A. JASAITIS, A. DIMITROV, T. PIOLOT, J. SALAMERO, Journal of Microscopy, 2015

Summary Multiconfocal microscopy gives a good compromise between fast imaging and reasonable resolution. However, the low intensity of live fluorescent emitters is a major limitation to this technique. Aberrations induced by the optical setup, especially the mismatch of the refractive index ...

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Aberration‐corrected STEM for atomic‐resolution imaging and analysis

04-May-2015 | O.L. Krivanek, T.C. Lovejoy, N. Dellby, Journal of Microscopy, 2015

Summary Aberration‐corrected scanning transmission electron microscopes are able to form electron beams smaller than 100 pm, which is about half the size of an average atom. Probing materials with such beams leads to atomic‐resolution images, electron energy loss and energy‐dispersive X‐ray ...

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[Special Issue Review] Transforming the optical landscape

01-May-2015 | J. B. Pendry; Yu Luo; Rongkuo Zhao, Science , 2015

Electromagnetism provides us with some of the most powerful tools in science, encompassing lasers, optical microscopes, magnetic resonance imaging scanners, radar, and a host of other techniques. To understand and develop the technology requires more than a set of formal equations. Scientists and ...

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Challenges of microtome‐based serial block‐face scanning electron microscopy in neuroscience

23-Apr-2015 | A. A. WANNER, M. A. KIRSCHMANN, C. GENOUD, Journal of Microscopy, 2015

Summary Serial block‐face scanning electron microscopy (SBEM) is becoming increasingly popular for a wide range of applications in many disciplines from biology to material sciences. This review focuses on applications for circuit reconstruction in neuroscience, which is one of the major ...

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A simple but precise method for quantitative measurement of the quality of the laser focus in a scanning optical microscope

10-Apr-2015 | J. Trägårdh, K. MacRae, C. Travis, R. Amor, G. Norris, S.H. Wilson, G.‐L. Oppo, G. McConnell, Journal of Microscopy, 2015

Summary We report a method for characterizing the focussing laser beam exiting the objective in a laser scanning microscope. This method provides the size of the optical focus, the divergence of the beam, the ellipticity and the astigmatism. We use a microscopic‐scale knife edge in the form of ...

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p‐Si/SnO2/Fe2O3 Core/Shell/Shell Nanowire Photocathodes for Neutral pH Water Splitting

21-Mar-2015 | Alireza Kargar, Sung Joo Kim, Paniz Allameh, Chulmin Choi, Namseok Park, Huisu Jeong, Yusin Pak, Gun Young Jung, Xia ..., Advanced Functional Materials, 2015

Silicon is one of the promising materials for solar water splitting and hydrogen production; however, it suffers from two key factors, including the large external potential required to drive water splitting reactions at its surface and its instability in the electrolyte. In this study, a ...

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Seamless stitching of tile scan microscope images

18-Mar-2015 | F.B. Legesse, O. Chernavskaia, S. Heuke, T. Bocklitz, T. Meyer, J. Popp, R. Heintzmann, Journal of Microscopy, 2015

Summary For diagnostic purposes, optical imaging techniques need to obtain high‐resolution images of extended biological specimens in reasonable time. The field of view of an objective lens, however, is often smaller than the sample size. To image the whole sample, laser scanning microscopes ...

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Formation of Multiple Structural Formats of DNA in a Bio‐Deep Eutectic Solvent

16-Mar-2015 | Chandrakant Mukesh, Kamalesh Prasad, Macromolecular Chemistry and Physics, 2015

A solution of DNA (salmon testes, 2.5% w/w) in a bio‐based deep eutectic solvent consisting of the mixture of choline chloride and ethylene glycol (Chol.Cl–EG 1:2) is turned to a soft ion gel upon addition of 6 n HCl (pH 2.9) at room temperature (25 °C). The gel structure is lost when 6 n NaOH ...

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