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Intrinsic Properties of Larval Zebrafish Neurons in Ethanol

03-05-2013 | Hiromi Ikeda et al., PLoS ONE, 2013

by Hiromi Ikeda, Alison H. Delargy, Tohei Yokogawa, Jason M. Urban, Harold A. Burgess, Fumihito Ono The behavioral effects of ethanol have been studied in multiple animal models including zebrafish. Locomotion of zebrafish larvae is resistant to high concentrations of ethanol in bath ...

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Development of a Plastic Embedding Method for Large-Volume and Fluorescent-Protein-Expressing Tissues

05-04-2013 | Zhongqin Yang et al., PLoS ONE, 2013

by Zhongqin Yang, Bihe Hu, Yuhui Zhang, Qingming Luo, Hui Gong Fluorescent proteins serve as important biomarkers for visualizing both subcellular organelles in living cells and structural and functional details in large-volume tissues or organs. However, current techniques for ...

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Enhanced Human Tissue Microdialysis Using Hydroxypropyl-ß-Cyclodextrin as Molecular Carrier

05-04-2013 | Marcus May et al., PLoS ONE, 2013

by Marcus May, Sandor Batkai, Alexander A. Zoerner, Dimitrios Tsikas, Jens Jordan, Stefan Engeli Microdialysis sampling of lipophilic molecules in human tissues is challenging because protein binding and adhesion to the membrane limit recovery. Hydroxypropyl-ß-cyclodextrin ...

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Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli

22-03-2013 | Dongchang Sun et al., PLoS ONE, 2013

by Dongchang Sun, Bing Wang, Lihong Zhu, Mengyao Chen, Linlin Zhan Our previous work established that DNA is naturally transferable on agar plates through a new transformation system which is regulated by the stationary phase master regulator RpoS in Escherichia coli. In this ...

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Dynamic Neural Network of Insight: A Functional Magnetic Resonance Imaging Study on Solving Chinese ‘Chengyu’ Riddles

15-03-2013 | Qingbai Zhao et al., PLoS ONE, 2013

by Qingbai Zhao, Zhijin Zhou, Haibo Xu, Shi Chen, Fang Xu, Wenliang Fan, Lei Han The key components of insight include breaking mental sets and forming the novel, task-related associations. The majority of researchers have agreed that the anterior cingulate cortex may mediate ...

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Solution Structure of the QUA1 Dimerization Domain of pXqua, the Xenopus Ortholog of Quaking

08-03-2013 | Muzaffar Ali et al., PLoS ONE, 2013

by Muzaffar Ali, R. William Broadhurst The STAR protein family member Quaking is essential for early development in vertebrates. For example, in oligodendrocyte cells it regulates the splicing, localization, translation and lifetime of a set of mRNAs that code for crucial ...

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The Center for Epidemiologic Studies Depression Scale: A Review with a Theoretical and Empirical Examination of Item Content and Factor Structure

01-03-2013 | R. Nicholas Carleton et al., PLoS ONE, 2013

by R. Nicholas Carleton, Michel A. Thibodeau, Michelle J. N. Teale, Patrick G. Welch, Murray P. Abrams, Thomas Robinson, Gordon J. G. Asmundson Background The Center for Epidemiologic Studies Depression Scale (CES-D; Radloff, 1977) is a commonly used freely available self-report measure of ...

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The Effectiveness of Internet Cognitive Behavioural Therapy (iCBT) for Depression in Primary Care: A Quality Assurance Study

22-02-2013 | Alishia D Williams et al., PLoS ONE, 2013

by Alishia D Williams, Gavin Andrews Background Depression is a common, recurrent, and debilitating problem and Internet delivered cognitive behaviour therapy (iCBT) could offer one solution. There are at least 25 controlled trials that demonstrate the efficacy of iCBT. The aim of the ...

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A New Family of Intrinsically Disordered Proteins: Structural Characterization of the Major Phasin PhaF from Pseudomonas putida KT2440

15-02-2013 | Beatriz Maestro et al., PLoS ONE, 2013

by Beatriz Maestro, Beatriz Galán, Carlos Alfonso, Germán Rivas, Maria A. Prieto, Jesús M. Sanz Phasins are intracellular polyhydroxyalkanoat4e (PHA)-associated proteins involved in the stabilization of these bacterial carbon storage granules. Despite its importance in PHA ...

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Structural and Functional Analysis of the DEAF-1 and BS69 MYND Domains

25-01-2013 | Fatiha Kateb et al., PLoS ONE, 2013

by Fatiha Kateb, Helene Perrin, Konstantinos Tripsianes, Peijian Zou, Roberta Spadaccini, Matthew Bottomley, Titus M. Franzmann, Johannes Buchner, Stephane Ansieau, Michael Sattler DEAF-1 is an important transcriptional regulator that is required for embryonic development and is ...

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