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Increase of SARS-CoV 3CL peptidase activity due to macromolecular crowding effects in the milieu composition

18-11-2010 | Debora N. Okamoto; Lilian C.G. Oliveira; Marcia Y. Kondo; Maria H.S. Cezari; Zoltán Szeltner; Tünde ..., Biological Chemistry, 2010

Abstract The 3C-like peptidase of the severe acute respiratory syndrome virus (SARS-CoV) is strictly required for viral replication, thus being a potential target for the development of antiviral agents. In contrast to monomeric picornavirus 3C peptidases, SARS-CoV 3CLpro exists in equilibrium ...

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Identification and evaluation of a new nucleic acid amplification test target for specific detection of Mycobacterium tuberculosis

18-10-2010 | Lianhua Qin; Ruijuan Zheng; Chao Fan; Jiangli Cai; Zhonghua Liu; Jie Wang; Junmei Lu; Ruiliang Jin; Hua Yan ..., Clinical Chemistry , 2010

Abstract Background: Accurate and early diagnosis of tuberculosis (TB) is of major importance in the management and control of TB. Because the conventional bacteriological diagnosis of TB has several limitations, nucleic acid amplification (NAA) tests have emerged as promising alternatives. A ...

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Small-angle X-ray and neutron scattering as a tool for structural systems biology

12-07-2010 | Dmitri I. Svergun, Biological Chemistry, 2010

Abstract Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad ...

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