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An efficient reagent for covalent introduction of alkyne into proteins

21-10-2014 | Jie Zhang; Dejun Ma; Dawei Du; Zhen Xi; Long Yi, Organic & Biomolecular Chemistry, 2014

A cheap and bench-stable reagent was synthesized for direct and covalent introduction of alkynes into tyrosine of target proteins, which can be further modified based on click reaction to achieve fluorescence labelling or PEGylation. This reagent should be a generally useful toolbox for chemical ...

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New Insights in the Structure-Spectrum Relationship in S65T/H148D and E222Q/H148D Green Fluorescent Protein Mutants. A Theoretical Assessment.

21-10-2014 | Pau Armengol; Ricard Gelabert; Miquel Moreno; José M Lluch, Organic & Biomolecular Chemistry, 2014

Green Fluorescent Protein (GFP) variant S65T/H148D recovers the A-band fluorescence lost in the single mutant S65T and it has been established that Asp148 is the alternate proton acceptor for the excited state proton transfer (ESPT). This mutant has been widely studied and presents unique ...

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Green synthesis of peptide-templated fluorescent copper nanoclusters for temperature sensing and cellular imaging

21-10-2014 | Hong Huang; hua Li; Ai-Jun Wang; Shuxian zhong; Keming Fang; Jiu-Ju Feng, Analyst, 2014

A simple and green approach was developed for preparation of fluorescent Cu nanoclusters (NCs) using artificial peptide CLEDNN as a template. The as-synthesized Cu NCs exhibited high fluorescence quantum yield (7.3%) and good stability, along with excitation and temperature dependent fluorescent ...

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Encoding through the host–guest structure: construction of multiplexed fluorescent beads

21-10-2014 | Ying Zhu; Hong Xu; Kaimin Chen; Jianping Fu; Hongchen Gu, Chemical Communication, 2014

A new encoding strategy based on the host–guest structure was developed, where encoding is achieved by combinational use of a set of guest particles with different fluorescence intensity levels at various ratios. This system was proven to be well-defined and highly controllable.

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Lighting up fluoride ions in cellular mitochondria using a highly selective and sensitive fluorescent probe

21-10-2014 | Shiling Zhang; Jiangli Fan; Shuangzhe Zhang; Jingyun Wang; Xianwu Wang; Jianjun Du; Xiaojun Peng, Chemical Communication, 2014

We report a highly selective and sensitive fluorescent probe (FP) for detecting fluoride ions, for the first time, lighting up the fluoride ions in mitochondria with a strong green fluorescence. FP could be easily prepared as fluoride paper test strips to detect fluoride ions in aqueous solutions ...

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Nanoscale characterization of DNA conformation using dual-color fluorescence axial localization and label-free biosensing

21-10-2014 | Xirui Zhang; George Daaboul; Philipp Sphuler; David Freedman; Abdulkadir Yurt; Sunmin Ahn; Oguzhan Avci; Selim Unlu, Analyst, 2014

Quantitative determination of the density and conformation of DNA molecules tethered to the surface can help optimize and understand DNA nanosensors and nanodevices, which use conformational or motional changes of surface-immobilized DNA for detection or actuation. We present an interferometric ...

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Strong fluorescence emission localized at a tapered silver-plated sub-wavelength pore

21-10-2014 | Jin-Lei Yang; Shuo-Hui Cao; Qian Liu; Shuang Zhao; Yu-Bin Zheng; Yao-Qun Li, New Journal of Chemistry, 2014

We observed up to 200 times fluorescence enhancement inside a tapered silver-plated sub-wavelength pore in comparison with the free space fluorescence. Fluorescence lifetime data was obtained to explore the strong fluorescence enhancement. The mechanism mainly arises from the silver film, the ...

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Silicon-substituted xanthene dyes and their applications to bioimaging

21-10-2014 | Yu Kushida; Tetsuo Nagano; kenjiro Hanaoka, Analyst, 2014

Fluorescence imaging is one of the most powerful techniques for visualizing temporal and spatial changes of biological phenomena in living cells, and many fluorescence probes have been developed. In particular, xanthene dyes such as fluorescein and rhodamines have favorable characteristics, such ...

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Screening and investigation of a cyanine fluorescence probe for simultaneous sensing of glutathione and cysteine with single excitation

21-10-2014 | Xu Wang; Jianzheng Lv; Xueying Yao; Yong Li; Fang Huang; Mengmeng Li; Jie Yang; Xiuyun Ruan; Bo Tang, Chemical Communication, 2014

Here we report the synthesis and screening of Cy-3-NO2 that showed simultaneous fluorescence sensing ability towards glutathione and cysteine with single excitation, and its application in living cell imaging.

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A simple and effective 1,2,3-triazole based “turn-on” fluorescence sensor for the detection of anions

21-10-2014 | Debanjana Ghosh; Shannon Rhodes; Karena Vontrell Hawkins; Domonique Winder; Austin Atkinson; Weihua Ming; Clifford P ..., New Journal of Chemistry, 2014

A novel and effective 1,2,3-triazole based fluorescence chemosensor has been synthesized for the specific detection of anions in homogeneous medium. Notably, the molecule, synthesized in one step using “Click chemistry”, is a simple 1,4-diaryl-1,2,3-triazole, containing a phenol moiety. The probe ...

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