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1,529 Newest Publications about the topic spectrometers

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Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism

17-May-2016 | Daniel James Wilkinson, Mass Spectrometry Reviews, 2016

Over a century ago, Frederick Soddy provided the first evidence for the existence of isotopes; elements that occupy the same position in the periodic table are essentially chemically identical but differ in mass due to a different number of neutrons within the atomic nucleus. Allied to the ...

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Mass measurement accuracy of the Orbitrap in intact proteome analysis

16-May-2016 | Fan Yu, Houqin Fang, Kaijie Xiao, Yan Liu, Bingbing Xue, Zhixin Tian, Rapid Communications in Mass Spectrometry, 2016

Rationale The mass measurement accuracy (MMA) of Orbitrap mass spectrometers is 1–5 ppm according to the manufacturer's specification; yet, up to 50 ppm has been used as mass tolerance to interpret Orbitrap data in the literature. A systematic evaluation of MMA is thus necessary to find the ...

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Low Charge and Reduced Mobility of Membrane Protein Complexes Has Implications for Calibration of Collision Cross Section Measurements

13-May-2016 | Timothy M. Allison; Michael Landreh; Justin L. P. Benesch; Carol V. Robinson, Analytical Chemistry, 2016

Ion mobility mass spectrometry of integral membrane proteins provides valuable insights into their architecture and stability. Here we show that, due to their lower charge, the average mobility of native-like membrane protein ions is approximately 30% lower than that of soluble proteins of ...

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How Suitable is Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight for Metabolite Imaging from Clinical Formalin-Fixed and Paraffin-Embedded Tissue Samples in Comparison to Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry?

02-May-2016 | Achim Buck; Benjamin Balluff; Andreas Voss; Rupert Langer; Horst Zitzelsberger; Michaela Aichler; Axel Walch, Analytical Chemistry, 2016

In research and clinical settings, formalin-fixed and paraffin-embedded (FFPE) tissue specimens are collected routinely and therefore this material constitutes a highly valuable source to gather insight in metabolic changes of diseases. Among mass spectrometry techniques to examine the molecular ...

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Authentication of Closely Related Fish and Derived Fish Products Using Tandem Mass Spectrometry and Spectral Library Matching

02-May-2016 | Merel A. Nessen; Dennis J. van der Zwaan; Sander Grevers; Hans Dalebout; Martijn Staats; Esther Kok; Magnus Palmblad, Journal of Agricultural and Food Chemistry, 2016

Proteomics methodology has seen increased application in food authentication, including tandem mass spectrometry of targeted species-specific peptides in raw, processed, or mixed food products. We have previously described an alternative principle that uses untargeted data acquisition and ...

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Characterization of a series of absolute isotope reference materials for magnesium: ab initio calibration of the mass spectrometers, and determination of isotopic compositions and relative atomic weights

20-Apr-2016 | Jochen Vogl; Björn Brandt; Janine Noordmann; Olaf Rienitz; Dmitriy Malinovskiy, Journal of Analytical Atomic Spectrometry, 2016

For the first time an ab initio calibration for absolute Mg isotope ratios was carried out, without any a priori assumptions made. All quantities influencing the calibration such as purity of the enriched isotopes or liquid and solid densities were carefully analysed and their associated ...

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Calibration procedure for secular frequency scanning in ion trap mass spectrometers

13-Apr-2016 | Dalton T. Snyder, Christopher J. Pulliam, R. Graham Cooks, Rapid Communications in Mass Spectrometry, 2016

Rationale Mass spectra can be recorded using ion traps by scanning the frequency of an alternating current (ac) signal that corresponds to the secular frequency of a trapped ion. There is a considerable simplification in the instrumentation needed to perform such a scan compared with ...

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Enhancement of Lateral Diffusion in Catanionic Vesicles during Multilamellar-to-Unilamellar Transition

11-Apr-2016 | S. Mitra; V. K. Sharma; V. Garcia-Sakai; A. Orecchini; T. Seydel; M. Johnson; R. Mukhopadhyay, Journal of Physical Chemistry B, 2016

Catanionic vesicles are formed spontaneously by mixing cationic and anionic dispersions in aqueous solution in suitable conditions. Because of spontaneity in formation, long-term stability, and easy modulation of size and charge, they have numerous advantages over conventional lipid-based ...

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Using Variable Ionization Energy Time-of-Flight Mass Spectrometry with Comprehensive GC×GC To Identify Isomeric Species

05-Apr-2016 | Mohammed S. Alam; Christopher Stark; Roy M. Harrison, Analytical Chemistry, 2016

Although GC×GC-ToF-MS allows the separation of thousands of peaks, many of these peaks are not positively identified owing to the lack of mass spectral library data and/or standard materials, leading to a substantial amount of information being inaccessible. The fragmentation patterns of ...

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Development and Evaluation of a Parallel Reaction Monitoring Strategy for Large-Scale Targeted Metabolomics Quantification

04-Apr-2016 | Juntuo Zhou; Huiying Liu; Yang Liu; Jia Liu; Xuyang Zhao; Yuxin Yin, Analytical Chemistry, 2016

Recent advances in mass spectrometers which have yielded higher resolution and faster scanning speeds have expanded their application in metabolomics of diverse diseases. Using a quadrupole-Orbitrap LC–MS system, we developed an efficient large-scale quantitative method targeting 237 metabolites ...

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