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 Courses
Biotechnology Workshop 200818 Sep 2008 - 19 Sep 2008
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Venue:
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Hochschule für Angewandte Wissenschaften Hamburg
Hamburg
Germany
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Category:
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Biotechnology; Laboratory Equipment; Kultivierung zu hohen Zelldichten (high cell density cultivation HCDC), Primäraufarbeitung, Laborbioreaktoren
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Organiser:
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Sartorius AG
Weender Landstraße 94-108
37075 Göttingen
Germany
Ms Dr. Dagmar Simons
Phone: +49 551 308 1227
Fax: +49 551 308 1293
http://www.sartorius.de/
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Description:
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High Cell Density Cultivation (HCDC) of Escherichia coli and Primary Downstream Processing (DSP)
Target group
Attendees include biologists, biochemists, chemists, biotechnologists, engineers and technical assistants with basic knowledge of microbiology, cultivation of microorganisms and of molecular biology, who are in charge of managing bioreactors or the downstream processing of bioprocess products or willing to do this in future.
Objectives
Emphasis is placed in Part I (#862021) on an efficient and easy way to carry out high cell density cultivation (HCDC), and on the safe handling of bench-scale bioreactors. This will be demonstrated in practical work as well as in computer simulations. Consideration is given to both a sufficient oxygen supply and fundamentals of the genetics of Escherichia coli and suitable vectors. General aspects of microbial cultivations and possible products will be discussed, including remarks on analytical methods supporting fermentation processes.
Part II (#862022) provides an introduction to primary downstream processing techniques, e.g. primary steps, to isolate the protein of interest, so-called products capturing.
Course content
* Introduction to a virtual bioreactor (guided simulation)
* Bench-scale bioreactors; handling from preparation to cell harvest
* Composition of media for HCDCs
* Strategies for cultivation and analysis
* Escherichia coli and vectors
* Separation strategies
- Cell harvest and clarification by
- - Centrifugation
- - Depth filtration (lenticular filtration)
- - Crossflow filtration (microfiltration)
- Cell disruption methods
- Purification by
- - Expanded bed adsorption
- - Membrane chromatography
- - Crossflow filtration (ultrafiltration) |
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Additional information:
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http://www.sartorius.de/index.php?id=1308&VID=75
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Additional information
 Sartorius AG
Göttingen, Germany
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