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Zetasizer Nano delivers fully automated protein characterization

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Malvern’s Zetasizer Nano is a powerful easy-to-use protein characterization tool that uses dynamic light scattering (DLS) to rapidly and accurately measure particle size. Offering exceptional sensitivity and accuracy, it allows measurement of the size and molecular weight of protein molecules as small as 1000 Daltons. Its high sensitivity and precision temperature control are critical factors that allow thermally dependent structural changes to be detected at very low volumes and concentrations.

Coupled with the MPT-2 Autotitrator, the Zetasizer Nano provides fully automated protein characterization, encompassing measurement of particle size, zeta potential and molecular weight.

The Zetasizer Nano is being used for applications including: optimizing protein crystallization conditions; measuring at true concentrations used in research environments; determining absolute molecular weight and polydispersity; assessing the importance of the second virial coefficient; and characterizing protein homogeneity by DLS.

The system has been designed to meet the low concentration and sample volume requirements in pharmaceutical and biomolecular applications, as well as the high concentration requirements for colloidal applications. Satisfying this unique mix of demands is achieved through the integration of a backscatter optical system and the design of a novel cell chamber. The MPT-2 Autotitrator facilitates automated pH, additive, dilution and conductivity titrations.

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