Identification and confirmation of unknown substances by hyphenation of HPTLC with MS

Rapid and contamination-free elution directly from the layer to your MS with the TLC-MS Interface 2

Through the pioneering concept of hyphenating high-performance thin-layer chromatography with mass spectrometry unequivocal substance identification is possible. The CAMAG TLC-MS Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Depending on the MS system, a substance can be identified within a minute via its mass spectrum, or for an unknown substance zone, the respective sum formula can be obtained. Furthermore, interesting zones can be eluted into vials for further investigations with, e.g. NMR, (ATR-)FTIR, ESI-MS, and MALDI-MS.

The chromatogram zones are eluted from the HPTLC plate with methanol or another suitable solvent with the flow speed appropriate for the LC-MS system. The round elution head is used for circular zones and the oval elution head for zones in the form of bands. After extraction the eluate is either transferred online to the mass spectrometer or collected in a sample vial for further offline analysis.

The CAMAG TLC-MS Interface 2 features a modified elution head and an easily accessible, exchangeable filter, arranged in front of the valve. Cleaning is facilitated as compared to the previous version, making it highly efficient. By pushing a button, the elution path is cleaned of matrix particles with compressed air, increasing the lifetime of the filter and preventing the system from becoming blocked. These filters can be easily replaced without any modification to the elution head.

Plate positioning is significantly simplified: together with the positioning scale and the adjustable plate-stopper, the integrated crosshair laser enables accurate and reproducible positioning of the plate.

Applications
Topics
Get in Touch with the Supplier Now
More about CAMAG
  • Videos

    Automated Spraying Device for Postchromatographic Derivatization of TLC Chromatograms

    With the Derivatizer CAMAG presents an automated spraying device which sets a new standard of reproducibility in the reagent transfer onto TLC plates by employing a unique "micro droplet" spraying technology. The CAMAG Derivatizer ensures homogeneous and reproducible application of all comm ... more

  • News

    CBS 105, CAMAG Bibliography Service

    The autumn issue of CBS is now available and as always, it’s free of charge.Each issue of CBS focuses on recent examples of planar chromatography in practice. Articles are topical and they come from a great variety of fields of interest. In this issue: One article comes from the field of o ... more

    Validated HPTLC Methods for Identification of Botanicals

    Identification of medicinal plants is traditionally one of the premier fields of application for TLC. When quality and safety became an issue, methods for identification of herbal drugs by TLC fingerprint were readily implemented into pharmacopoeias all over the world. Recently the USP, the ... more

    CAMAG Bibliography Service

    The new CAMAG Bibliography Service CBS 96 with over 100 abstracts of recent publications, demonstrating the flexibility of Thin-Layer Chromatography, has been released. CBS 96 puts emphasis on efficient methods for food control. Featured is for example a method for Quantification of ITX in ... more

  • Companies

    CAMAG Chemieerzeugnisse & Adsorptionstechnik AG

    CAMAG has a tradition of support for Thin-Layer Chromatography since 1961. We develop and manufacture simple as well as sophisticated instruments and associated software for all steps of TLC: -Sample Application -Chromatogram Development -Derivatization -Evaluation -Documentation We pr ... more

    CAMAG Chemie-Erzeugnisse & Adsorptionstechnik AG & Co. GmbH

    more