Centrifugal partition chromatography (CPC) also known as counter current chromatography (CCC), is a liquid-liquid purification technique that does not require traditional solid supports. This silica-free technology isolates the maximum amount of a specific molecule at the highest purity quickly and without using a silica column or any support media.
KEY BENEFITS OF CPC PURIFICATION
- No column or silica to replace
- Five times less solvent consumption
- High injection capacity (from mg to kg of sample)
- 95% recovery with high purity, no irreversible absorption or denaturation of fragile molecule
- Complex sample injection/purification in one step
- Adaptable to diverse application fields, such as from complex natural extracts, protein and synthetic molecule purification, and fermentation broth.
WHY CHOOSE CPC SYSTEMS?
- A variety of configuration options are available: all CPC systems can be connected to either a PLC Purification System or Gilson modular HPLC system in place of a traditional LC column
- Entirely automated solution with dedicated Gilson Glider CPC software when configured with a PLC Purification System
- Targeted detection via UV/VIS, ELSD and MS. Mass-directed purification saves time and money by targeting compounds of interest, which results in a reduction of the overall number of fractions collected that require dry down, reconstitution and analysis.
Classic LC columns consist of a cylinder with inlet and outlet at each end, filled with different sized silica gel. The CPC column also has an entry and exit for the mobile phase, but these are the only similarities with an HPLC column. The CPC system is based on a liquid-liquid technique. A special design and the creation of a centrifugal field makes it possible to keep one phase of the two-phase system in the column.
The column consists in principle of several discs on a rotor. These discs have more than a thousand cells, which are connected by a thin channel (see close-up). The rotor or column is kept under a homogeneous centrifugal field. This allows, due to the specific geometry of the cells, the retention of the stationary phase.
A four-way valve allows a change in the direction of elution. You can either work in ascending mode (see scheme) (light phase = mobile phase) or in descending mode (heavy phase = mobile phase). In this way, it is possible to work in both normal and inverted modes without replacing the column.