Take Full Advantage of Spectral Properties of Dielectric Interference Filters

Precise and software-controlled – ideal for fluorescence microscopy

Optical filters and beam splitters are crucial components of fluorescence microscopes. In order to obtain accurate assay results, the spectral properties must be optimally adjusted to the fluorescence application and dyes. This has been done so far by manual and often cumbersome replacing of the filter components.

Precise Tuning of Spectral Properties with the TuneCube

The TuneCube from AHF analysentechnik allows to rotate dielectric filters precisely and software-controlled to adjust their spectral properties perfectly to the specific application. It is also possible to use the transmitted and the reflected light simultaneously! Thus, regular bandpass or edge filters can be used as beam splitters. This makes the TuneCube a versatile auxiliary device for your fluorescence microscope!

Functioning of the TuneCube

The interference filter is placed into the TuneCube and can there be rotated by a software-controlled motor until the desired setting is achieved. The physical behavior of dielectric interference filters builds the basis for it. In general, all light that’s not transmitted by the filter will be reflected. Moreover, dielectric filters change their spectral properties with the angle of incident to the incoming beam as the effective layer thickness of the thin film changes. Thus, it can be adjusted spectrally by rotating the filter. The spectrum changes towards short wavelength ranges with increasing angle of incidence.

Benefits of the TuneCube

  • The TuneCube mechanism ensures the reflected part of the light is always at 90 ° to the incident beam, regardless of the selected rotation angle of the filter.
  • Regular bandpass or edge filters can be used as beam splitters.
  • The blocking properties also remain stable in an angle range of 0−60 ° (depending on the filter type).
  • The reflected beam shows no beam offset and the transmitted beam only very small offset when rotating of the filter.
  • The dimensions are only 13 x 13 x 13 cm which makes it a compact and space-saving unit

A Versatile Device

The TuneCube preserves the spectral properties of the filter which allows to perform 2D wide-field recordings, e.g. dynamic image-splitting for FRET or Ca2+ imaging.

It can also be used as a tunable beam splitter for tunable lasers with
appropriate signal transmission through the filter (e.g. in Raman spectroscopy)
as well as for far-field hyperspectral imaging with very high transmission of
the signal (> 90%).

Two Models Are Available

The fully motorized and software-controllable model of the TuneCube allows to rotate the filter between 5° and 55° (<1 sec.). It is equipped with various interfaces for controlling.

The reduced version of the TuneCube works with a manual rotation mechanism which makes the unit smaller and the price more favorable. Filters can be rotated between 7° and 38°.

The TuneCube offers various and interesting possibilities to get more out of your fluorescence microscope. Call us today and get expert advice from our team!

  • beam splitters

  • interference filters

  • filters

  • optical filters

  • microscope Accessories

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