Nano-Macro-Proteins – Asymmetric Flow Field-Flow Fractionation Platform

Separation of Proteins, Polymers and Nanoparticles Without a Column

The new Postnova AF2000MF Flow FFF is a temperature controlled FFF-MALS-System and perfectly suitable for separation, characterization and fractionation of proteins, bio-/polymers and nanoparticles. The complete system allows the user to use temperature as another dimension for FFF separation. So Postnova Analytics presents another important innovation in the area of Field-Flow Fractionation. The separation in FFF is based simply on the diffusion of parts (brownian molecular movement) and a temperature control brings up a large variety of advantages. The new AF2000MF series offers such a temperature control and therefore reaches a new level of performance.

The System shows a higher resolution, reproducibility and recovery as comparable FFF setups. The AF2000MF connected to a MALS-light scattering detector is a completely new developed and optimized analytical system based on Asymmetric Flow Field-Flow Fractionation (AF4) and Multi-Angle Light Scattering (MALS). The separation range covers a very broad molar mass and particles size range. The separation itself is a very gentle procedure without any shear forces. The online connected and completely in the AF4 software integrated Multi-Angle Light Scattering-Detector makes it possible to measure the molar mass and the particles size directly without the usage of calibration standards.

The AF2000MF-MALS series has a technically similar setup like a GPC-MALS-System but avoids the common limitations of chromatography. So the in liquid chromatography used column is in an AF2000MF-MALS system replaced by a specially patented separation channel who has no stationary phase. The retention happens only based on size and by the influence of a cross flow as a separation force.

Proteins, antibody, aggregates, conjugates, viruses, liposomes, starches, hyaluronic acids, alginates and many other biopolymers but also a large variety of nanoparticles like gold, carbon-nano materials and latex particles, can be separated in a range of 10E3 - 10E9 and 1 nm - 10 µm. In contrast to column chromatography the Mid Temperature Flow FFF has no size exclusion limit which would reduce the separation range for high and ultra high molar masses. Through the absence of any stationary phase and free choice of the solvent any unwanted adsorption effects can be avoided.

Unique Advantages

  • Ultrahigh resolution and very broad size range for polymers and particles
  • No shear forces and gentle separation especially for biomolecules
  • Direct molar mass and particle size determination without calibration standards
  • Temperature controlled system with patented reusable separation channel
  • No stationary phase or columns as spare part necessary
  • Analytes can be directly fractionated, collected and measure with additional on- and offline analytical methods as fluorescence, EM, AFM, etc.

The Postnova AF2000MF-MALS system consists of the modular FFF separation system and the integrated into the software connected MALS-/UV- and RI-Detectors. The unique Nova FFF software platform controls the complete FFF detector system. But the software also collects the data and directly calculates the molecular weight and the particle size. Additional detectors as ICP-MS, viscometry, DAD, fluorescence and ELSD can be also connected.

With the presentation of the temperature controlled asymmetrical Flow FFF connected to MALS detector Postnova has achieved a new mile stone in improving the Flow-FFF-MALS technology. With this innovation the technique can be presented to a large variety of users in the area of biopharma, polymers and nanotechnology as a routine system. The AF2000MF-MALS series is a basic tool now for any researcher and quality assurance manager, who has to solve questions in the area of proteins, biopolymers and nanoparticles. The technology has the special advantage of a very broad separation range which can never be achieved with classical chromatographic methods combined with a high separation technology for proteins, bio-/polymers and nanoparticles. This allows the separation of these analytes within one sample with a higher resolution and characterization. Additionally the common disadvantages of packed columns are avoided. These features make the Postnova AF2000MF-MALS series to the ideal tool in the area of protein, bio-/polymer and nanoparticle characterization.

  • Postnova Analytics

  • FFF systems

  • AF4 systems

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