With the Derivatizer CAMAG presents an automated spraying device which sets a new standard of reproducibility in the reagent transfer onto TLC plates by employing a unique "micro droplet" spraying technology. The CAMAG Derivatizer ensures homogeneous and reproducible application of all common reagents. To meet the diverging physicochemical properties of the different reagents, e.g. acidity or viscosity, four different color-coded spray nozzles are employed, and the user can select from six spraying modes.
In addition to the significantly increased homogeneous reagent distribution, the Derivatizer offers other advantages compared to manual spraying:
- Environmentally friendly and safe handling through a closed system
- Intuitive handling and easy cleaning
- Low reagent consumption through efficient operation (4 ml for 20 x 20 cm plates and 2 ml for 20 x 10 cm plates)
Derivatization means another step in the process, consequently causing an increase in the variance. If an experienced technician manually sprays, the relative standard deviation of the measured values significantly rises up to 12.0%. In contrast with the CAMAG Derivatizer, the standard deviation slightly increases between 2.5 and 4.5%. Moreover, these results are user-independent and reproducible.