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Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole–Imidazole Polyamide Probes

Synthetic molecules that bind sequence-specifically to DNA have been developed for varied biological applications, including anticancer activity, regulation of gene expression, and visualization of specific genomic regions. Increasing the number of base pairs targeted by synthetic molecules strengthens their sequence specificity. Our group has been working on the development of pyrrole–imidazole polyamides that bind to the minor groove of DNA in a sequence-specific manner without causing denaturation. Recently, we reported a simple synthetic method of fluorescent tandem dimer polyamide probes composed of two hairpin moieties with a linking hinge, which bound to 12 bp in human telomeric repeats (5′-(TTAGGG)n-3′) and could be used to specifically visualize telomeres in chemically fixed cells under mild conditions. We also performed structural optimization and extension of the target base pairs to allow more specific staining of telomeres. In the present study, we synthesized tandem tetramer polyamides compo...

Authors:   Yusuke Kawamoto; Asuka Sasaki; Anandhakumar Chandran; Kaori Hashiya; Satoru Ide; Toshikazu Bando; Kazuhiro Maeshima; Hiroshi Sugiyama
Journal:   Journal of the American Chemical Society
Year:   2016
DOI:   10.1021/jacs.6b09023
Publication date:   13-Oct-2016
Facts, background information, dossiers
  • DNA
  • gene expression
  • cells
More about American Chemical Society Publications
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