my.chemeurope.com
With an accout for my.chemeurope.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
A primary goal of metabolomics is the characterization of a potentially very large number of metabolites that are part of complex mixtures. Application to biofluids and tissue samples offers insights into biochemical metabolic pathways and their role in health and disease. 1D 1H and 2D 13C‐1H HSQC NMR spectra are most commonly used for this purpose. They yield quantitative information about each proton of the mixture, but not which protons belong to the same molecule. Interpretation requires the use of NMR spectral databases naturally limited to known metabolites. Here, we present a new method that uses complementary ion exchange resin beads to differentially attenuate 2D NMR cross‐peaks that belong to different metabolites. Based on their characteristic attenuation patterns, cross‐peaks could be clustered and assigned to individual molecules, including unknown metabolites with multiple spin systems, as demonstrated for a metabolite model mixture and E. coli cell lysate.
| Authors: | Jiaqi Yuan, Bo Zhang, Rafael Bruschweiler | |
| Journal: | Chemistry - A European Journal | |
| Year: | 2017 | |
| Pages: | n/a | |
| DOI: | 10.1002/chem.201701572 | |
| Publication date: | 19-May-2017 |
The unfolding landscape of the congenital myasthenic syndromes
Abstract Congenital myasthenic syndromes (CMS) are heterogeneous disorders in which the safety margin of neuromuscular transmission is impaired by one or more specific mechanisms. Since the advent of next‐generation sequencing methods, the discovery of novel CMS targets and phenotypes ha ... more
Passive transfer models of myasthenia gravis with muscle‐specific kinase antibodies
Abstract Myasthenia gravis (MG) with antibodies to muscle‐specific kinase (MuSK) is characterized by fluctuating fatigable weakness. In MuSK MG, involvement of bulbar muscles, neck, and shoulder and respiratory weakness are more prominent than in acetylcholine receptor (AChR) MG. MuSK au ... more
Abstract Because of the failure of many promising therapeutics identified in preclinical evaluation, funding sources have established guidelines for increased rigor in animal evaluations. The myasthenia gravis (MG) community of scientists has developed guidelines for preclinical assessme ... more
Smart skin made of recyclable materials may transform medicine and robotics
Smart skin that can respond to external stimuli could have important applications in medicine and robotics. Using only items found in a typical household, researchers have created multi-sensor artificial skin that's capable of sensing pressure, temperature, humidity, proximity, pH, and air ... more
How mercury contamination affects reptiles in the Amazon basin
Mercury contamination in water and on land is of worldwide concern due to its toxic effects on ecosystems and human health. Mercury toxicity is of particular concern to reptiles because they are currently experiencing population declines. Also, reptiles are ideal indicators of mercury conta ... more
Bioengineers Succeed in Producing Plastic Without the use of Fossil Fuels
A team of pioneering South Korean scientists have succeeded in producing the polymers used for everyday plastics through bioengineering, rather than through the use of fossil fuel based chemicals. This groundbreaking research, which may now allow for the production of environmentally consci ... more
Read what you need to know about our industry portal chemeurope.com.
© 1997-2022 LUMITOS AG, All rights reserved
