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Immobilized monolithic enzymatic reactors and its application on analysis of In‐Vitro Fertilization media samples

Classical proteomics approaches involve enzymatic hydrolysis of proteins (either separated by polyacrylamide gels or in solution) followed by peptide identification using LC‐MS/MS analysis. This method requires normally more than sixteen hours to complete. In the case of clinical analysis, it is of the utmost importance to provide fast and reproducible analysis with minimal manual sample handling. Herein we report the method development for online protein digestion on immobilized monolithic enzymatic reactors (IMER) to accelerate protein digestion, reduce manual sample handling, and provide reproducibility to the digestion process in clinical laboratory. An integrated online digestion and separation method using monolithic immobilized enzymatic reactor was developed and applied to digestion and separation of in‐vitro‐fertilization media.

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Authors:   Wei‐Qiang Chen, Philipp Obermayr, Urh Černigoj, Jana Vidič, Tanja Panić‐Janković, Goran Mitulović
Journal:   ELECTROPHORESIS
Year:   2017
Pages:   n/a
DOI:   10.1002/elps.201700197
Publication date:   14-Jun-2017
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