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A Printed Equilibrium Dialysis Device with Integrated Membranes for Improved Binding Affinity Measurements

Equilibrium dialysis is a simple and effective technique used for investigating the binding of small molecules and ions to proteins. A three-dimensional (3D) printer was used to create a device capable of measuring binding constants between a protein and a small ion based on equilibrium dialysis. Specifically, the technology described here enables the user to customize an equilibrium dialysis device to fit their own experiments by choosing membranes of various material and molecular-weight cutoff values. The device has dimensions similar to that of a standard 96-well plate, thus being amenable to automated sample handlers and multichannel pipettes. The device consists of a printed base that hosts multiple windows containing a porous regenerated-cellulose membrane with a molecular-weight cutoff of ∼3500 Da. A key step in the fabrication process is a print-pause-print approach for integrating membranes directly into the windows subsequently inserted into the base. The integrated membranes display no leaking...

Authors:   Cody W. Pinger; Andrew A. Heller; Dana M. Spence
Journal:   Analytical Chemistry
Year:   2017
DOI:   10.1021/acs.analchem.7b01848
Publication date:   06-Jul-2017
Facts, background information, dossiers
  • small molecules
  • proteins
  • Binding constants
More about American Chemical Society Publications
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