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Development and Validation of Quantitative Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry Assay for Anticoagulant Rodenticides in Liver

Anticoagulant rodenticides (ARs) are used to control rodent populations; however, exposure to nontarget animals occurs. A sensitive and rugged quantitative method was developed, optimized, and validated for eight ARs in liver. Target analytes comprised two chemical classes: hydroxycoumarins (warfarin, coumachlor, dicoumarol, bromadiolone, brodifacoum, and difethialone) and indanediones (diphacinone and chlorophacinone). In this method, liver extracts were cleaned using dispersive solid phase extraction (d-SPE) to remove matrix interferences and analyzed by reverse phase ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). Electrospray ionization in negative ion mode combined with multiple reaction monitoring (MRM) using a triple quadrupole mass spectrometer provided simultaneous confirmation and quantitation. Detection limits spanned 0.75–25 ng/g, and lower quantitation limits were established as 50 ng/g. Interassay method accuracy ranged from 92 to 110% across the analytical rang...

Authors:   Lori L. Smith; Boying Liang; Marcia C. Booth; Michael S. Filigenzi; Andriy Tkachenko; Cynthia L. Gaskill
Journal:   Journal of Agricultural and Food Chemistry
Year:   2017
DOI:   10.1021/acs.jafc.7b02280
Publication date:   27-Jul-2017
Facts, background information, dossiers
  • matrix Interferences
  • electrospray ionization
  • animals
More about American Chemical Society Publications
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