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A Flow‐through Column Electrolytic Cell for Supercritical Fluid Chromatography#

A novel flow‐through column electrolytic cell was proposed as a detector to obtain current signals for supercritical fluid chromatography. The electrochemical cell consisted of two electrodes and its holder, and a working and a counter electrode were fabricated from 192 carbon strings, which were composed of 400 carbon fibers of 10 μm in diameter filled into a heat‐shrinkable tube. These electrodes were placed in the center of a holder made from polyether ether ketone blocks and they were separated by polytetrafluoroethylene membrane filters. To evaluate the sensitivity of this cell, a standard solution of ferrocene was injected into the supercritical fluid chromatography system connected to the electrolytic cell. The ferrocene was eluted through a silica gel column using a mixture of a mobile phase of supercritical CO2 and a modifier of methanol containing ammonium acetate. The current peak area of ferrocene correlated to the ferrocene concentration in the range of 10–400 μmol/L (r = 0.999). Moreover, the limit of detection on the column estimated from a signal‐to‐noise ratio of 3 was 9.8 × 10−13 mol.

This article is protected by copyright. All rights reserved

Authors:   Kazuhiro Yamamoto, Tatsuya Ueki, Naoyuki Higuchi, Kouji Takahashi, Akira Kotani, Hideki Hakamata
Journal:   Journal of Separation Science
Year:   2017
Pages:   n/a
DOI:   10.1002/jssc.201700317
Publication date:   17-Aug-2017
Facts, background information, dossiers
  • solution
  • silica
  • polyether ether ketone
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