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Inhibition of fermentation evolution in bread doughs for aroma analyses


The inhibition of the residual fermentation in bread doughs, in order to avoid its evolution, could be crucial to achieve reliable qualitative aroma analyses. Several options have emerged up until now, but they present some drawbacks. In this study, a mixture of methyl octanoate and methyl decanoate (Fames) has been suggested as a non‐toxic alternative to the traditional use of mercuric chloride (HgCl2). Rheofermentometric analyses revealed that although HgCl2 is quicker, Fames solution is highly effective in less than 20 min. Moreover, when HgCl2 was added to 90 min fermented dough, it exhibited an unexpected behavior with a high release of CO2 without the generation of ethyl alcohol, which could affect the dough structure. SHS‐GC/MS analyses of ethyl alcohol and 2/3‐methyl‐1‐butanol corroborated the rheofermentometer's results, with a visible reduction in the peak areas and significant differences in the One‐way Anova between Fames doughs and blank doughs. The application of the Fames solution to SPME‐GC/MS‐QTof analyses involved a reduction in the areas regarding the blank without interferences, showing a logical progression of the volatile compounds over the fermentation time, increasing their concentration from 0 to 90 min. This progression was normally lost when the inhibitors were not added, since the yeast acted in an uncontrolled manner due to the changes of temperature during freezing, thawing or chromatographic analyses, leading to wrong aroma results.

  • The Fames solution (20 g/L of methyl octanoate/methyl decanoate in dimethyl sulfide) represents an effective, non‐toxic alternative to HgCI2 for inhibiting the fermentation evolution (peak areas are visible decreased), achieving accurate aroma analyses of doughs.
  • When the residual fermentation is not inhibited, the yeasts act uncontrolled and there is no logical evolution of the SPME‐GC/QT of peak areas of the volatile compounds during fermentation.
  • However, when it is inhibited, there is a logical increase in the SPME‐GC/QT of peak areas of the volatile compounds from 0 min to 90 min of fermentation.

Authors:   Joana Pico, José Bernal, María Jesús Nozal, Manuel Gómez
Journal:   Flavour and Fragrance Journal
Year:   2017
Pages:   n/a
DOI:   10.1002/ffj.3405
Publication date:   07-Sep-2017
Facts, background information, dossiers
  • fermentation
  • volatile compounds
  • solution
  • reductions
  • evolution
  • yeasts
  • yeasts
  • temperature
  • inhibitors
  • concentration
  • alcohol
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