my.chemeurope.com
With an accout for my.chemeurope.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
Mass spectrometry-based footprinting is an emerging approach for studying protein structure. Because integral membrane proteins are difficult targets for conventional structural biology, we recently developed a mass spectrometry (MS) footprinting method to probe membrane protein–drug interactions in live cells. This method can detect structural differences between apo and drug-bound states of membrane proteins, with the changes inferred from MS quantification of the cysteine modification pattern, generated by residue-specific chemical labeling. Here, we describe the experimental design, interpretation, advantages, and limitations of using cysteine footprinting by taking as an example the interaction of warfarin with vitamin K epoxide reductase, a human membrane protein. Compared with other structural methods, footprinting of proteins in live cells produces structural information for the near native state. Knowledge of cellular conformational states is a necessary complement to the high-resolution structur...
| Authors: | Guomin Shen; Shuang Li; Weidong Cui; Shixuan Liu; Yihu Yang; Michael Gross; Weikai Li | |
| Journal: | Biochemistry | |
| Year: | 2017 | |
| DOI: | 10.1021/acs.biochem.7b00874 | |
| Publication date: | 20-Dec-2017 |
We recently generalized the formerly alignment-dependent multivariate image analysis applied to quantitative structure–activity relationships (MIA-QSAR) method through the application of the discrete Fourier transform (DFT), allowing for its application to noncongruent and structurally dive ... more
Proteomic Analysis of the Downstream Signaling Network of PARP1
Poly-ADP-ribosylation (PARylation) is a protein posttranslational modification (PTM) that is critically involved in many biological processes that are linked to cell stress responses. It is catalyzed by a class of enzymes known as poly-ADP-ribose polymerases (PARPs). In particular, PARP1 is ... more
Oxygen Activation and Energy Conservation by Cytochrome c Oxidase
This review focuses on the type A cytochrome c oxidases (CcO), which are found in all mitochondria and also in several aerobic bacteria. CcO catalyzes the respiratory reduction of dioxygen (O2) to water by an intriguing mechanism, the details of which are fairly well understood today as a r ... more
Rohm and Haas Powder Coatings is delighted to announce that its Reading, Pennsylvania, facility is the first site in the world to receive the RCMS® Site Certification awarded by the American Chemical Council (ACC). When Raj Gupta, Chairman and CEO of Rohm and Haas Company, became Chairman ... more
American Chemical Society Publications
To advance the broader chemistry enterprise and its practitioners for the benefit of Earth and its people. more
Read what you need to know about our industry portal chemeurope.com.
© 1997-2021 LUMITOS AG, All rights reserved
