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Laterally and Temporally Controlled Intracellular Staining by Light‐Triggered Release of Encapsulated Fluorescent Markers

Abstract

Fluorescent molecular markers were encapsulated. The capsules were additionally modified with plasmonic nanoparticles. The encapsulated markers were endocytosed by cells. Upon light stimulation the plasmonic nanoparticles generated heat, which opened the encapsulation and transiently perforated the endosomal/lysosomal membrane surrounding the capsule, thus allowing for release of the marker into the cytosol. Fluorescence labeling of different intracellular compartments was demonstrated in this way. Most important, the cells do not need to be fixed and perforated, as the molecular markers are introduced into cells by endocytosis and subsequent light‐induced release. Thus this technique allows for intracellular fluorescence labeling of living cells.

Lighting up single cells: Encapsulated molecular markers can be intracellularly released upon an external light trigger and stain intracellular structures in living cells (see figure). Importantly, the cells do not need to be fixed and perforated, as the molecular markers are introduced into cells by endocytosis.

Authors:   Karsten Kantner, Joanna Rejman, Karl V. L. Kraft, Mahmoud G. Soliman, Mikhail V. Zyuzin, Alberto Escudero, Pablo del Pino, Wolfgang J. Parak
Journal:   Chemistry - A European Journal
Year:   2018
Pages:   n/a
DOI:   10.1002/chem.201706135
Publication date:   17-Jan-2018
Facts, background information, dossiers
  • endocytosis
  • encapsulation
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