my.chemeurope.com
With an accout for my.chemeurope.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
High-lateral-resolution secondary ion mass spectrometry (SIMS) has the potential to provide functional and depth resolved information from small biological structures, such as viral particles (virions) and phage, but sputter rate and sensitivity are not characterized at shallow depths relevant to these structures. Here we combine stable isotope labeling of the DNA of vaccinia virions with correlated SIMS imaging depth profiling and atomic force microscopy (AFM) to develop a nonlinear, nonequilibrium sputter rate model for the virions and validate the model on the basis of reconstructing the location of the DNA within individual virions. Our experiments with a Cs+ beam show an unexpectedly high initial sputter rate (∼100 um2·nm·pA–1·s–1) with a rapid decline to an asymptotic rate of 0.7 um2·nm·pA–1·s–1 at an approximate depth of 70 nm. Correlated experiments were also conducted with glutaraldehyde-fixed virions, as well as O– and Ga+ beams, yielding similar results. Based on our Cs+ sputter rate model, the...
| Authors: | Sean D. Gates; Richard C. Condit; Nissin Moussatche; Benjamin J. Stewart; Alexander J. Malkin; Peter K. Weber | |
| Journal: | Analytical Chemistry | |
| Year: | 2018 | |
| DOI: | 10.1021/acs.analchem.7b02786 | |
| Publication date: | 19-Jan-2018 |
We recently generalized the formerly alignment-dependent multivariate image analysis applied to quantitative structure–activity relationships (MIA-QSAR) method through the application of the discrete Fourier transform (DFT), allowing for its application to noncongruent and structurally dive ... more
Proteomic Analysis of the Downstream Signaling Network of PARP1
Poly-ADP-ribosylation (PARylation) is a protein posttranslational modification (PTM) that is critically involved in many biological processes that are linked to cell stress responses. It is catalyzed by a class of enzymes known as poly-ADP-ribose polymerases (PARPs). In particular, PARP1 is ... more
Oxygen Activation and Energy Conservation by Cytochrome c Oxidase
This review focuses on the type A cytochrome c oxidases (CcO), which are found in all mitochondria and also in several aerobic bacteria. CcO catalyzes the respiratory reduction of dioxygen (O2) to water by an intriguing mechanism, the details of which are fairly well understood today as a r ... more
Rohm and Haas Powder Coatings is delighted to announce that its Reading, Pennsylvania, facility is the first site in the world to receive the RCMS® Site Certification awarded by the American Chemical Council (ACC). When Raj Gupta, Chairman and CEO of Rohm and Haas Company, became Chairman ... more
American Chemical Society Publications
To advance the broader chemistry enterprise and its practitioners for the benefit of Earth and its people. more
Read what you need to know about our industry portal chemeurope.com.
© 1997-2022 LUMITOS AG, All rights reserved
